Rhea - reaction knowledgebase

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Name^help_outline a ribonucleotide in RNA Identifier RHEA-COMP:15261 Reactive part ^help_outline
- Name ^help_outline ribonucleotide residue Identifier CHEBI:90675 Charge -1 Formula C5H7O6PR SMILES^help_outline *[C@@H]1O[C@H](COP(=O)(*)[O-])[C@@H](O*)[C@H]1O 2D coordinates Mol file for the small molecule Search links Involved in 227 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Search links Involved in 1 reaction(s) Find proteins in UniProtKB for this molecule
Name ^help_outline S-adenosyl-L-methionine Identifier CHEBI:59789 Charge 1 Formula C15H23N6O5S InChIKey^help_outline MEFKEPWMEQBLKI-AIRLBKTGSA-O SMILES^help_outline C[S+](CC[C@H]([NH3+])C([O-])=O)C[C@H]1O[C@H]([C@H](O)[C@@H]1O)n1cnc2c(N)ncnc12 2D coordinates Mol file for the small molecule Search links Involved in 868 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Name^help_outline a 2'-O-methylribonucleotide in RNA Identifier RHEA-COMP:15262 Reactive part ^help_outline
- Name ^help_outline 2'-O-methylribonucleotide residue Identifier CHEBI:90676 Charge -1 Formula C6H9O6PR SMILES^help_outline *[C@@H]1O[C@H](COP(=O)(*)[O-])[C@@H](O*)[C@H]1OC 2D coordinates Mol file for the small molecule Search links Involved in 3 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Search links Involved in 1 reaction(s) Find proteins in UniProtKB for this molecule
Name ^help_outline H⁺ Identifier CHEBI:15378 Charge 1 Formula H InChIKey^help_outline GPRLSGONYQIRFK-UHFFFAOYSA-N SMILES^help_outline [H+] 2D coordinates Mol file for the small molecule Search links Involved in 9,431 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Name ^help_outline S-adenosyl-L-homocysteine Identifier CHEBI:57856 Charge 0 Formula C14H20N6O5S InChIKey^help_outline ZJUKTBDSGOFHSH-WFMPWKQPSA-N SMILES^help_outline Nc1ncnc2n(cnc12)[C@@H]1O[C@H](CSCC[C@H]([NH3+])C([O-])=O)[C@@H](O)[C@H]1O 2D coordinates Mol file for the small molecule Search links Involved in 792 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule

Cross-references

	RHEA:58956	RHEA:58957	RHEA:58958	RHEA:58959
Reaction direction	undefined	left-to-right	right-to-left	bidirectional
UniProtKB ^help_outline	1 proteins	1 proteins
Gene Ontology ^help_outline	GO:0062105

Related reactions ^help_outline

Specific form(s) of this reaction

RHEA:67027
a 5'-end (N⁷-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleoside)-(ribonucleotide) in mRNA + S-adenosyl-L-methionine <=> a 5'-end (N⁷-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleoside)-(2'-O-methyl-ribonucleotide) in mRNA + H⁺ + S-adenosyl-L-homocysteine
RHEA:67023
a 5'-end (N⁷-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L-methionine <=> a 5'-end (N⁷-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleoside) in mRNA + H⁺ + S-adenosyl-L-homocysteine
RHEA:65383
a 5'-end (5'-triphosphoguanosine)-adenylyl-adenylyl-cytidylyl-adenosine in mRNA + S-adenosyl-L-methionine <=> a 5'-end (5'-triphosphoguanosine)-(2'-O-methyladenylyl)-adenylyl-cytidylyl-adenosine in mRNA + H⁺ + S-adenosyl-L-homocysteine
RHEA:56887
adenosine³² in tRNA + S-adenosyl-L-methionine <=> 2'-O-methyladenosine³² in tRNA + H⁺ + S-adenosyl-L-homocysteine
RHEA:48631
a ribonucleotide in rRNA + S-adenosyl-L-methionine <=> a 2'-O-methylribonucleotide in rRNA + H⁺ + S-adenosyl-L-homocysteine
RHEA:43199
adenosine⁴ in tRNA^His + S-adenosyl-L-methionine <=> 2'-O-methyladenosine⁴ in tRNA^His + H⁺ + S-adenosyl-L-homocysteine
RHEA:43103
S-adenosyl-L-methionine + uridine⁴⁴ in tRNA^Ser <=> 2'-O-methyluridine⁴⁴ in tRNA^Ser + H⁺ + S-adenosyl-L-homocysteine
RHEA:43087
cytidine³⁴ in tRNA + S-adenosyl-L-methionine <=> 2'-O-methylcytidine³⁴ in tRNA + H⁺ + S-adenosyl-L-homocysteine
RHEA:42971
cytidine⁵⁶ in tRNA + S-adenosyl-L-methionine <=> 2'-O-methylcytidine⁵⁶ in tRNA + H⁺ + S-adenosyl-L-homocysteine
RHEA:42939
S-adenosyl-L-methionine + uridine³² in tRNA <=> 2'-O-methyluridine³² in tRNA + H⁺ + S-adenosyl-L-homocysteine
RHEA:42935
cytidine³² in tRNA + S-adenosyl-L-methionine <=> 2'-O-methylcytidine³² in tRNA + H⁺ + S-adenosyl-L-homocysteine
RHEA:42727
guanosine²⁹²² in 27S pre-rRNA + S-adenosyl-L-methionine <=> 2'-O-methylguanosine²⁹²² in 27S pre-rRNA + H⁺ + S-adenosyl-L-homocysteine
RHEA:32770
cytidine⁴ in tRNA^Pro + S-adenosyl-L-methionine <=> 2'-O-methylcytidine⁴ in tRNA^Pro + H⁺ + S-adenosyl-L-homocysteine
RHEA:20080
guanosine¹⁸ in tRNA + S-adenosyl-L-methionine <=> 2'-O-methylguanosine¹⁸ in tRNA + H⁺ + S-adenosyl-L-homocysteine

Publications

FTSJ3 is an RNA 2'-O-methyltransferase recruited by HIV to avoid innate immune sensing.

Ringeard M., Marchand V., Decroly E., Motorin Y., Bennasser Y.

In mammals, 2'-O-methylation of RNA is a molecular signature by which the cellular innate immune system distinguishes endogenous from exogenous messenger RNA1-3. However, the molecular functions of RNA 2'-O-methylation are not well understood. Here we have purified TAR RNA-binding prote ... >> More

In mammals, 2'-O-methylation of RNA is a molecular signature by which the cellular innate immune system distinguishes endogenous from exogenous messenger RNA1-3. However, the molecular functions of RNA 2'-O-methylation are not well understood. Here we have purified TAR RNA-binding protein (TRBP) and its interacting partners and identified a DICER-independent TRBP complex containing FTSJ3, a putative 2'-O-methyltransferase (2'O-MTase). In vitro and ex vivo experiments show that FTSJ3 is a 2'O-MTase that is recruited to HIV RNA through TRBP. Using RiboMethSeq analysis4, we identified predominantly FTSJ3-dependent 2'-O-methylations at specific residues on the viral genome. HIV-1 viruses produced in FTSJ3 knockdown cells show reduced 2'-O-methylation and trigger expression of type 1 interferons (IFNs) in human dendritic cells through the RNA sensor MDA5. This induction of IFN-α and IFN-β leads to a reduction in HIV expression. We have identified an unexpected mechanism used by HIV-1 to evade innate immune recognition: the recruitment of the TRBP-FTSJ3 complex to viral RNA and its 2'-O-methylation. << Less

Nature 565:500-504(2019) [PubMed] [EuropePMC]

RHEA:58959 a ribonucleotide in RNA + S-adenosyl-L-methionine <=> a 2'-O-methylribonucleotide in RNA + H(+) + S-adenosyl-L-homocysteine Reaction with equal flow from both directions. help_outline

Reaction participants Show >> << Hide

Cross-references

Related reactions help_outline

Specific form(s) of this reaction

Publications

FTSJ3 is an RNA 2'-O-methyltransferase recruited by HIV to avoid innate immune sensing.

RHEA:58959 a ribonucleotide in RNA + S-adenosyl-L-methionine <=> a 2'-O-methylribonucleotide in RNA + H(+) + S-adenosyl-L-homocysteine Reaction with equal flow from both directions. ^help_outline

Related reactions ^help_outline