Enzymes
UniProtKB help_outline | 1 proteins |
Reaction participants Show >> << Hide
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Namehelp_outline
a ribonucleotide in RNA
Identifier
RHEA-COMP:15261
Reactive part
help_outline
- Name help_outline ribonucleotide residue Identifier CHEBI:90675 Charge -1 Formula C5H7O6PR SMILEShelp_outline *[C@@H]1O[C@H](COP(=O)(*)[O-])[C@@H](O*)[C@H]1O 2D coordinates Mol file for the small molecule Search links Involved in 227 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline S-adenosyl-L-methionine Identifier CHEBI:59789 Charge 1 Formula C15H23N6O5S InChIKeyhelp_outline MEFKEPWMEQBLKI-AIRLBKTGSA-O SMILEShelp_outline C[S+](CC[C@H]([NH3+])C([O-])=O)C[C@H]1O[C@H]([C@H](O)[C@@H]1O)n1cnc2c(N)ncnc12 2D coordinates Mol file for the small molecule Search links Involved in 868 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
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Namehelp_outline
a 2'-O-methylribonucleotide in RNA
Identifier
RHEA-COMP:15262
Reactive part
help_outline
- Name help_outline 2'-O-methylribonucleotide residue Identifier CHEBI:90676 Charge -1 Formula C6H9O6PR SMILEShelp_outline *[C@@H]1O[C@H](COP(=O)(*)[O-])[C@@H](O*)[C@H]1OC 2D coordinates Mol file for the small molecule Search links Involved in 3 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline H+ Identifier CHEBI:15378 Charge 1 Formula H InChIKeyhelp_outline GPRLSGONYQIRFK-UHFFFAOYSA-N SMILEShelp_outline [H+] 2D coordinates Mol file for the small molecule Search links Involved in 9,431 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline S-adenosyl-L-homocysteine Identifier CHEBI:57856 Charge 0 Formula C14H20N6O5S InChIKeyhelp_outline ZJUKTBDSGOFHSH-WFMPWKQPSA-N SMILEShelp_outline Nc1ncnc2n(cnc12)[C@@H]1O[C@H](CSCC[C@H]([NH3+])C([O-])=O)[C@@H](O)[C@H]1O 2D coordinates Mol file for the small molecule Search links Involved in 792 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Cross-references
RHEA:58956 | RHEA:58957 | RHEA:58958 | RHEA:58959 | |
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Reaction direction help_outline | undefined | left-to-right | right-to-left | bidirectional |
UniProtKB help_outline |
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Gene Ontology help_outline |
Related reactions help_outline
Specific form(s) of this reaction
- RHEA:67025
- RHEA:67021
- RHEA:65381
- RHEA:56885
- RHEA:48629
- RHEA:43197
- RHEA:43101
- RHEA:43085
- RHEA:42969
- RHEA:42937
- RHEA:42933
- RHEA:42725
- RHEA:32768
- RHEA:20078
Publications
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FTSJ3 is an RNA 2'-O-methyltransferase recruited by HIV to avoid innate immune sensing.
Ringeard M., Marchand V., Decroly E., Motorin Y., Bennasser Y.
In mammals, 2'-O-methylation of RNA is a molecular signature by which the cellular innate immune system distinguishes endogenous from exogenous messenger RNA<sup>1-3</sup>. However, the molecular functions of RNA 2'-O-methylation are not well understood. Here we have purified TAR RNA-binding prote ... >> More
In mammals, 2'-O-methylation of RNA is a molecular signature by which the cellular innate immune system distinguishes endogenous from exogenous messenger RNA<sup>1-3</sup>. However, the molecular functions of RNA 2'-O-methylation are not well understood. Here we have purified TAR RNA-binding protein (TRBP) and its interacting partners and identified a DICER-independent TRBP complex containing FTSJ3, a putative 2'-O-methyltransferase (2'O-MTase). In vitro and ex vivo experiments show that FTSJ3 is a 2'O-MTase that is recruited to HIV RNA through TRBP. Using RiboMethSeq analysis<sup>4</sup>, we identified predominantly FTSJ3-dependent 2'-O-methylations at specific residues on the viral genome. HIV-1 viruses produced in FTSJ3 knockdown cells show reduced 2'-O-methylation and trigger expression of type 1 interferons (IFNs) in human dendritic cells through the RNA sensor MDA5. This induction of IFN-α and IFN-β leads to a reduction in HIV expression. We have identified an unexpected mechanism used by HIV-1 to evade innate immune recognition: the recruitment of the TRBP-FTSJ3 complex to viral RNA and its 2'-O-methylation. << Less