Reaction participants Show >> << Hide
- Name help_outline L-ribulose 5-phosphate Identifier CHEBI:58226 Charge -2 Formula C5H9O8P InChIKeyhelp_outline FNZLKVNUWIIPSJ-CRCLSJGQSA-L SMILEShelp_outline OCC(=O)[C@@H](O)[C@@H](O)COP([O-])([O-])=O 2D coordinates Mol file for the small molecule Search links Involved in 4 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline H2O Identifier CHEBI:15377 (CAS: 7732-18-5) help_outline Charge 0 Formula H2O InChIKeyhelp_outline XLYOFNOQVPJJNP-UHFFFAOYSA-N SMILEShelp_outline [H]O[H] 2D coordinates Mol file for the small molecule Search links Involved in 6,485 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline L-ribulose Identifier CHEBI:16880 (CAS: 2042-27-5) help_outline Charge 0 Formula C5H10O5 InChIKeyhelp_outline ZAQJHHRNXZUBTE-UCORVYFPSA-N SMILEShelp_outline C(O)C(=O)[C@@H](O)[C@@H](O)CO 2D coordinates Mol file for the small molecule Search links Involved in 5 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline phosphate Identifier CHEBI:43474 Charge -2 Formula HO4P InChIKeyhelp_outline NBIIXXVUZAFLBC-UHFFFAOYSA-L SMILEShelp_outline OP([O-])([O-])=O 2D coordinates Mol file for the small molecule Search links Involved in 1,029 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Cross-references
| RHEA:79819 | RHEA:79820 | RHEA:79821 | RHEA:79822 | |
|---|---|---|---|---|
| Reaction direction help_outline | undefined | left-to-right | right-to-left | bidirectional |
| UniProtKB help_outline |
|
Publications
-
Removal of phosphoglycolate in hyperthermophilic archaea.
Michimori Y., Izaki R., Su Y., Fukuyama Y., Shimamura S., Nishimura K., Miwa Y., Hamakita S., Shimosaka T., Makino Y., Takeno R., Sato T., Beppu H., Cann I., Kanai T., Nunoura T., Atomi H.
Many organisms that utilize the Calvin-Benson-Bassham (CBB) cycle for autotrophic growth harbor metabolic pathways to remove and/or salvage 2-phosphoglycolate, the product of the oxygenase activity of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco). It has been presumed that the occurren ... >> More
Many organisms that utilize the Calvin-Benson-Bassham (CBB) cycle for autotrophic growth harbor metabolic pathways to remove and/or salvage 2-phosphoglycolate, the product of the oxygenase activity of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco). It has been presumed that the occurrence of 2-phosphoglycolate salvage is linked to the CBB cycle, and in particular, the C2 pathway to the CBB cycle and oxygenic photosynthesis. Here, we examined 2-phosphoglycolate salvage in the hyperthermophilic archaeon <i>Thermococcus kodakarensis</i>, an obligate anaerobe that harbors a Rubisco that functions in the pentose bisphosphate pathway. <i>T. kodakarensis</i> harbors enzymes that have the potential to convert 2-phosphoglycolate to glycine and serine, and their genes were identified by biochemical and/or genetic analyses. 2-phosphoglycolate phosphatase activity increased 1.6-fold when cells were grown under microaerobic conditions compared to anaerobic conditions. Among two candidates, TK1734 encoded a phosphatase specific for 2-phosphoglycolate, and the enzyme was responsible for 80% of the 2-phosphoglycolate phosphatase activity in <i>T. kodakarensis</i> cells. The TK1734 disruption strain displayed growth impairment under microaerobic conditions, which was relieved upon addition of sodium sulfide. In addition, glycolate was detected in the medium when <i>T. kodakarensis</i> was grown under microaerobic conditions. The results suggest that <i>T. kodakarensis</i> removes 2-phosphoglycolate via a phosphatase reaction followed by secretion of glycolate to the medium. As the Rubisco in <i>T. kodakarensis</i> functions in the pentose bisphosphate pathway and not in the CBB cycle, mechanisms to remove 2-phosphoglycolate in this archaeon emerged independent of the CBB cycle. << Less
Proc Natl Acad Sci U S A 121:e2311390121-e2311390121(2024) [PubMed] [EuropePMC]
This publication is cited by 21 other entries.