Enzymes
| UniProtKB help_outline | 1 proteins |
Reaction participants Show >> << Hide
- Name help_outline 2-naphthyl sulfate Identifier CHEBI:167170 Charge -1 Formula C10H7O4S InChIKeyhelp_outline HXEZIDSFDHEIIQ-UHFFFAOYSA-M SMILEShelp_outline [O-]S(=O)(=O)OC1=CC=C2C=CC=CC2=C1 2D coordinates Mol file for the small molecule Search links Involved in 2 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline phenol Identifier CHEBI:15882 (Beilstein: 969616; CAS: 108-95-2) help_outline Charge 0 Formula C6H6O InChIKeyhelp_outline ISWSIDIOOBJBQZ-UHFFFAOYSA-N SMILEShelp_outline Oc1ccccc1 2D coordinates Mol file for the small molecule Search links Involved in 24 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline 2-naphthol Identifier CHEBI:10432 (Beilstein: 742134; CAS: 135-19-3) help_outline Charge 0 Formula C10H8O InChIKeyhelp_outline JWAZRIHNYRIHIV-UHFFFAOYSA-N SMILEShelp_outline OC1=CC2=CC=CC=C2C=C1 2D coordinates Mol file for the small molecule Search links Involved in 2 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline phenyl sulfate Identifier CHEBI:85289 Charge -1 Formula C6H5O4S InChIKeyhelp_outline CTYRPMDGLDAWRQ-UHFFFAOYSA-M SMILEShelp_outline [O-]S(=O)(=O)Oc1ccccc1 2D coordinates Mol file for the small molecule Search links Involved in 2 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Cross-references
| RHEA:79267 | RHEA:79268 | RHEA:79269 | RHEA:79270 | |
|---|---|---|---|---|
| Reaction direction help_outline | undefined | left-to-right | right-to-left | bidirectional |
| UniProtKB help_outline |
|
Related reactions help_outline
More general form(s) of this reaction
Publications
-
Molecular cloning of the arylsulfate sulfotransferase gene and characterization of its product from Enterobacter amnigenus AR-37.
Kwon A.-R., Oh T.-G., Kim D.-H., Choi E.-C.
The gene encoding the Enterobacter amnigenus AR-37 arylsulfate sulfotransferase (ASST) was cloned, sequenced, and expressed in Escherichia coli NM522. Sequencing led to the identification of three contiguous open reading frames (ORFs) on the same strand. Based on amino acid sequence homology, ORF1 ... >> More
The gene encoding the Enterobacter amnigenus AR-37 arylsulfate sulfotransferase (ASST) was cloned, sequenced, and expressed in Escherichia coli NM522. Sequencing led to the identification of three contiguous open reading frames (ORFs) on the same strand. Based on amino acid sequence homology, ORF1, ORF2, and ORF3 are designated astA, dsbA, and dsbB, respectively. A multiple sequence alignment revealed conserved regions in ASST. An N-terminal amino acid sequence analysis of the purified ASST from E. coli NM522 (pEAST72) showed that it is subject to N-terminal processing. The specific activity of purified ASST is 436.5 U/mg of protein. The enzyme is a monomeric protein with a molecular mass of 64 kDa. Using phenol as an acceptor substrate, 4-methylumbelliferyl sulfate is the best donor substrate, followed by beta-naphthyl sulfate, p-nitrophenyl sulfate (PNS), and alpha-naphthyl sulfate. For PNS, alpha-naphthol is the best acceptor substrate, followed by phenol, resorcinol, p-acetaminophen, tyramine, and tyrosine. The enzyme has a different acceptor specificity than the enzyme purified from Eubacterium A-44. It is similar to Klebsiella K-36 and Haemophilus K-12. The apparent K(m) values for PNS using phenol as an acceptor and for phenol using PNS as a donor are 0.163 and 0.314 mM, respectively. The pI and optimum pH are 6.1 and 9.0, respectively. << Less
Protein Expr. Purif. 17:366-372(1999) [PubMed] [EuropePMC]
This publication is cited by 1 other entry.