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- Name help_outline adenylyl MoO3-molybdopterin cofactor Identifier CHEBI:231441 Charge -4 Formula C20H22MoN10O15P2S2 InChIKeyhelp_outline ZDGVBQQZOIGKBT-NKWKRPJXSA-J SMILEShelp_outline C=12C(=O)NC(N)=NC1N[C@]3([C@@](N2)(C4=C([C@@H](COP(=O)(OP(=O)(OC[C@@H]5[C@H]([C@H]([C@H](N6C=7N=CN=C(C7N=C6)N)O5)O)O)[O-])[O-])O3)S[Mo-2](S4)(=O)(=O)=O)[H])[H] 2D coordinates Mol file for the small molecule Search links Involved in 2 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline adenylyl MoO2(OH)-molybdopterin cofactor Identifier CHEBI:231442 Charge -4 Formula C20H22MoN10O15P2S2 InChIKeyhelp_outline OOQOEPNVFLIVOM-NKWKRPJXSA-J SMILEShelp_outline C=12C(=O)NC(N)=NC1N[C@]3([C@@](N2)(C4=C([C@@H](COP(=O)(OP(=O)(OC[C@@H]5[C@H]([C@H]([C@H](N6C=7N=CN=C(C7N=C6)N)O5)O)O)[O-])[O-])O3)S[Mo-](S4)(=O)(=O)[O-])[H])[H] 2D coordinates Mol file for the small molecule Search links Involved in 2 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Cross-references
| RHEA:79031 | RHEA:79032 | RHEA:79033 | RHEA:79034 | |
|---|---|---|---|---|
| Reaction direction help_outline | undefined | left-to-right | right-to-left | bidirectional |
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Related reactions help_outline
More general form(s) of this reaction
Publications
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Mechanism of molybdate insertion into pterin-based molybdenum cofactors.
Probst C., Yang J., Krausze J., Hercher T.W., Richers C.P., Spatzal T., Kc K., Giles L.J., Rees D.C., Mendel R.R., Kirk M.L., Kruse T.
The molybdenum cofactor (Moco) is found in the active site of numerous important enzymes that are critical to biological processes. The bidentate ligand that chelates molybdenum in Moco is the pyranopterin dithiolene (molybdopterin, MPT). However, neither the mechanism of molybdate insertion into ... >> More
The molybdenum cofactor (Moco) is found in the active site of numerous important enzymes that are critical to biological processes. The bidentate ligand that chelates molybdenum in Moco is the pyranopterin dithiolene (molybdopterin, MPT). However, neither the mechanism of molybdate insertion into MPT nor the structure of Moco prior to its insertion into pyranopterin molybdenum enzymes is known. Here, we report this final maturation step, where adenylated MPT (MPT-AMP) and molybdate are the substrates. X-ray crystallography of the Arabidopsis thaliana Mo-insertase variant Cnx1E S269D D274S identified adenylated Moco (Moco-AMP) as an unexpected intermediate in this reaction sequence. X-ray absorption spectroscopy revealed the first coordination sphere geometry of Moco trapped in the Cnx1E active site. We have used this structural information to deduce a mechanism for molybdate insertion into MPT-AMP. Given their high degree of structural and sequence similarity, we suggest that this mechanism is employed by all eukaryotic Mo-insertases. << Less
Nat Chem 13:758-765(2021) [PubMed] [EuropePMC]
This publication is cited by 3 other entries.