Reaction participants Show >> << Hide
- Name help_outline N-acetyl-D-galactosamine Identifier CHEBI:28037 (Beilstein: 1429037; CAS: 14215-68-0,1811-31-0) help_outline Charge 0 Formula C8H15NO6 InChIKeyhelp_outline OVRNDRQMDRJTHS-KEWYIRBNSA-N SMILEShelp_outline CC(=O)N[C@H]1C(O)O[C@H](CO)[C@H](O)[C@@H]1O 2D coordinates Mol file for the small molecule Search links Involved in 21 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline O2 Identifier CHEBI:15379 (CAS: 7782-44-7) help_outline Charge 0 Formula O2 InChIKeyhelp_outline MYMOFIZGZYHOMD-UHFFFAOYSA-N SMILEShelp_outline O=O 2D coordinates Mol file for the small molecule Search links Involved in 2,709 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline H2O2 Identifier CHEBI:16240 (Beilstein: 3587191; CAS: 7722-84-1) help_outline Charge 0 Formula H2O2 InChIKeyhelp_outline MHAJPDPJQMAIIY-UHFFFAOYSA-N SMILEShelp_outline [H]OO[H] 2D coordinates Mol file for the small molecule Search links Involved in 449 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline N-acetyl-D-galactosamino-1,5-lactone Identifier CHEBI:184299 Charge 0 Formula C8H13NO6 InChIKeyhelp_outline NELQYZRSPDCGRQ-MVIOUDGNSA-N SMILEShelp_outline C1(=O)[C@H](NC(=O)C)[C@@H](O)[C@@H](O)[C@H](O1)CO 2D coordinates Mol file for the small molecule Search links Involved in 2 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Cross-references
| RHEA:69516 | RHEA:69517 | RHEA:69518 | RHEA:69519 | |
|---|---|---|---|---|
| Reaction direction help_outline | undefined | left-to-right | right-to-left | bidirectional |
| UniProtKB help_outline |
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Publications
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Discovery of two novel oxidases using a high-throughput activity screen.
Rembeza E., Boverio A., Fraaije M.W., Engqvist M.
Discovery of novel enzymes is a challenging task, yet a crucial one, due to their increasing relevance as chemical catalysts and biotechnological tools. In our work we present a high-throughput screening approach to discovering novel activities. A screen of 96 putative oxidases with 23 substrates ... >> More
Discovery of novel enzymes is a challenging task, yet a crucial one, due to their increasing relevance as chemical catalysts and biotechnological tools. In our work we present a high-throughput screening approach to discovering novel activities. A screen of 96 putative oxidases with 23 substrates led to the discovery of two new enzymes. The first enzyme, N-acetyl-D-hexosamine oxidase (EC 1.1.3.29) from Ralstonia solanacearum, is a vanillyl alcohol oxidase-like flavoprotein displaying the highest activity with N-acetylglucosamine and N-acetylgalactosamine. Before our discovery of the enzyme, its activity was an orphan one - experimentally characterized but lacking the link to amino acid sequence. The second enzyme, from an uncultured marine euryarchaeota, is a long-chain alcohol oxidase (LCAO, EC 1.1.3.20) active with a range of fatty alcohols, with 1-dodecanol being the preferred substrate. The enzyme displays no sequence similarity to previously characterised LCAOs, and thus is a completely novel representative of a protein with such activity. << Less
ChemBioChem 0:0-0(2021) [PubMed] [EuropePMC]
This publication is cited by 6 other entries.
Comments
RHEA:69516 part of RHEA:69512 Published in: "Purification and properties of N-acyl-D-hexosamine oxidase from Pseudomonas sp 15-1." Horiuchi, T. Agric. Biol. Chem. 53 (1989) 361–368. DOI:10.1080/00021369.1989.10869298 https://www.tandfonline.com/doi/abs/10.1080/00021369.1989.10869298