Reaction participants Show >> << Hide
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Namehelp_outline
a cytidine in 26S rRNA
Identifier
RHEA-COMP:17065
Reactive part
help_outline
- Name help_outline CMP residue Identifier CHEBI:82748 Charge -1 Formula C9H11N3O7P SMILEShelp_outline Nc1ccn([C@@H]2O[C@H](COP([O-])(-*)=O)[C@@H](O-*)[C@H]2O)c(=O)n1 2D coordinates Mol file for the small molecule Search links Involved in 66 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline S-adenosyl-L-methionine Identifier CHEBI:59789 Charge 1 Formula C15H23N6O5S InChIKeyhelp_outline MEFKEPWMEQBLKI-AIRLBKTGSA-O SMILEShelp_outline C[S+](CC[C@H]([NH3+])C([O-])=O)C[C@H]1O[C@H]([C@H](O)[C@@H]1O)n1cnc2c(N)ncnc12 2D coordinates Mol file for the small molecule Search links Involved in 868 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
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Namehelp_outline
a 5-methylcytidine in 26S rRNA
Identifier
RHEA-COMP:17066
Reactive part
help_outline
- Name help_outline 5-methylcytidine 5'-phosphate residue Identifier CHEBI:74483 Charge -1 Formula C10H13N3O7P SMILEShelp_outline C1=C(C(=NC(N1[C@@H]2O[C@H](COP(*)(=O)[O-])[C@H]([C@H]2O)O*)=O)N)C 2D coordinates Mol file for the small molecule Search links Involved in 35 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline H+ Identifier CHEBI:15378 Charge 1 Formula H InChIKeyhelp_outline GPRLSGONYQIRFK-UHFFFAOYSA-N SMILEShelp_outline [H+] 2D coordinates Mol file for the small molecule Search links Involved in 9,431 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline S-adenosyl-L-homocysteine Identifier CHEBI:57856 Charge 0 Formula C14H20N6O5S InChIKeyhelp_outline ZJUKTBDSGOFHSH-WFMPWKQPSA-N SMILEShelp_outline Nc1ncnc2n(cnc12)[C@@H]1O[C@H](CSCC[C@H]([NH3+])C([O-])=O)[C@@H](O)[C@H]1O 2D coordinates Mol file for the small molecule Search links Involved in 792 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Cross-references
RHEA:66588 | RHEA:66589 | RHEA:66590 | RHEA:66591 | |
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Reaction direction help_outline | undefined | left-to-right | right-to-left | bidirectional |
UniProtKB help_outline |
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Related reactions help_outline
More general form(s) of this reaction
Publications
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Methylation of ribosomal RNA by NSUN5 is a conserved mechanism modulating organismal lifespan.
Schosserer M., Minois N., Angerer T.B., Amring M., Dellago H., Harreither E., Calle-Perez A., Pircher A., Gerstl M.P., Pfeifenberger S., Brandl C., Sonntagbauer M., Kriegner A., Linder A., Weinhaeusel A., Mohr T., Steiger M., Mattanovich D., Rinnerthaler M., Karl T., Sharma S., Entian K.D., Kos M., Breitenbach M., Wilson I.B., Polacek N., Grillari-Voglauer R., Breitenbach-Koller L., Grillari J.
Several pathways modulating longevity and stress resistance converge on translation by targeting ribosomal proteins or initiation factors, but whether this involves modifications of ribosomal RNA is unclear. Here, we show that reduced levels of the conserved RNA methyltransferase NSUN5 increase th ... >> More
Several pathways modulating longevity and stress resistance converge on translation by targeting ribosomal proteins or initiation factors, but whether this involves modifications of ribosomal RNA is unclear. Here, we show that reduced levels of the conserved RNA methyltransferase NSUN5 increase the lifespan and stress resistance in yeast, worms and flies. Rcm1, the yeast homologue of NSUN5, methylates C2278 within a conserved region of 25S rRNA. Loss of Rcm1 alters the structural conformation of the ribosome in close proximity to C2278, as well as translational fidelity, and favours recruitment of a distinct subset of oxidative stress-responsive mRNAs into polysomes. Thus, rather than merely being a static molecular machine executing translation, the ribosome exhibits functional diversity by modification of just a single rRNA nucleotide, resulting in an alteration of organismal physiological behaviour, and linking rRNA-mediated translational regulation to modulation of lifespan, and differential stress response. << Less
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The ribosomal RNA m5C methyltransferase NSUN-1 modulates healthspan and oogenesis in Caenorhabditis elegans.
Heissenberger C., Rollins J.A., Krammer T.L., Nagelreiter F., Stocker I., Wacheul L., Shpylovyi A., Tav K., Snow S., Grillari J., Rogers A.N., Lafontaine D.L., Schosserer M.
Our knowledge about the repertoire of ribosomal RNA modifications and the enzymes responsible for installing them is constantly expanding. Previously, we reported that NSUN-5 is responsible for depositing m<sup>5</sup>C at position C2381 on the 26S rRNA in <i>Caenorhabditis elegans</i>. Here, we s ... >> More
Our knowledge about the repertoire of ribosomal RNA modifications and the enzymes responsible for installing them is constantly expanding. Previously, we reported that NSUN-5 is responsible for depositing m<sup>5</sup>C at position C2381 on the 26S rRNA in <i>Caenorhabditis elegans</i>. Here, we show that NSUN-1 is writing the second known 26S rRNA m<sup>5</sup>C at position C2982. Depletion of <i>nsun-1</i> or <i>nsun-5</i> improved thermotolerance and slightly increased locomotion at midlife, however, only soma-specific knockdown of <i>nsun-1</i> extended lifespan. Moreover, soma-specific knockdown of <i>nsun-1</i> reduced body size and impaired fecundity, suggesting non-cell-autonomous effects. While ribosome biogenesis and global protein synthesis were unaffected by <i>nsun-1</i> depletion, translation of specific mRNAs was remodeled leading to reduced production of collagens, loss of structural integrity of the cuticle, and impaired barrier function. We conclude that loss of a single enzyme required for rRNA methylation has profound and highly specific effects on organismal development and physiology. << Less