Reaction participants Show >> << Hide
- Name help_outline dGMP Identifier CHEBI:57673 (Beilstein: 7349077,3575246) help_outline Charge -2 Formula C10H12N5O7P InChIKeyhelp_outline LTFMZDNNPPEQNG-KVQBGUIXSA-L SMILEShelp_outline Nc1nc2n(cnc2c(=O)[nH]1)[C@H]1C[C@H](O)[C@@H](COP([O-])([O-])=O)O1 2D coordinates Mol file for the small molecule Search links Involved in 15 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline dGTP Identifier CHEBI:61429 Charge -4 Formula C10H12N5O13P3 InChIKeyhelp_outline HAAZLUGHYHWQIW-KVQBGUIXSA-J SMILEShelp_outline Nc1nc2n(cnc2c(=O)[nH]1)[C@H]1C[C@H](O)[C@@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)O1 2D coordinates Mol file for the small molecule Search links Involved in 18 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline dGDP Identifier CHEBI:58595 (Beilstein: 11523263) help_outline Charge -3 Formula C10H12N5O10P2 InChIKeyhelp_outline CIKGWCTVFSRMJU-KVQBGUIXSA-K SMILEShelp_outline Nc1nc2n(cnc2c(=O)[nH]1)[C@H]1C[C@H](O)[C@@H](COP([O-])(=O)OP([O-])([O-])=O)O1 2D coordinates Mol file for the small molecule Search links Involved in 13 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Cross-references
| RHEA:62144 | RHEA:62145 | RHEA:62146 | RHEA:62147 | |
|---|---|---|---|---|
| Reaction direction help_outline | undefined | left-to-right | right-to-left | bidirectional |
| UniProtKB help_outline |
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Related reactions help_outline
More general form(s) of this reaction
Publications
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A novel nucleotide kinase encoded by gene 1.7 of bacteriophage T7.
Tran N.Q., Lee S.J., Richardson C.C., Tabor S.
Gene 1.7 of bacteriophage T7 confers sensitivity of both phage T7 and its host Escherichia coli to dideoxythymidine (ddT). We have purified the product of gene 1.7, gp1.7. It exists in two forms of molecular weight 22,181 and 17,782. Only the C-terminal half of the protein is required to confer dd ... >> More
Gene 1.7 of bacteriophage T7 confers sensitivity of both phage T7 and its host Escherichia coli to dideoxythymidine (ddT). We have purified the product of gene 1.7, gp1.7. It exists in two forms of molecular weight 22,181 and 17,782. Only the C-terminal half of the protein is required to confer ddT sensitivity. We show that gp1.7 catalyses the phosphorylation of dGMP and dTMP to dGDP and dTDP, respectively, by using either GTP, dGTP or dTTP as the phosphate donor. Either form of gp1.7 exhibit identical kinase activity as compared with wild-type gp1.7 that contains a mixture of both forms. The K(m) of 70 microM and Kcat of 4.3 s(-1) for dTMP are similar to those found for E. coli thymidylate kinase. However, unlike the host enzyme, gp1.7 efficiently catalyses the conversion of the chain-terminating dideoxythymidylate (ddTMP) to ddTDP. This finding explains the sensitivity of phage T7 but not E. coli to exogenous ddT. Gp1.7 is unusual in that it has no sequence homology to any known nucleotide kinase, it has no identifiable nucleotide-binding motif and its activity is independent of added metal ions. When coupled with nucleoside diphosphate kinase, gp1.7 exponentially converts dTMP to dTTP. << Less
Mol. Microbiol. 77:492-504(2010) [PubMed] [EuropePMC]
This publication is cited by 4 other entries.