Reaction participants Show >> << Hide
- Name help_outline amicoumacin A Identifier CHEBI:144858 Charge 1 Formula C20H30N3O7 InChIKeyhelp_outline DCPWYLSPIAHJFU-YKRRISCLSA-O SMILEShelp_outline C1(=CC=CC2=C1C(O[C@@](C2)([C@@H](NC([C@H]([C@H]([C@H](CC(N)=O)[NH3+])O)O)=O)CC(C)C)[H])=O)O 2D coordinates Mol file for the small molecule Search links Involved in 2 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline ATP Identifier CHEBI:30616 (Beilstein: 3581767) help_outline Charge -4 Formula C10H12N5O13P3 InChIKeyhelp_outline ZKHQWZAMYRWXGA-KQYNXXCUSA-J SMILEShelp_outline Nc1ncnc2n(cnc12)[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]1O 2D coordinates Mol file for the small molecule Search links Involved in 1,280 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline ADP Identifier CHEBI:456216 (Beilstein: 3783669) help_outline Charge -3 Formula C10H12N5O10P2 InChIKeyhelp_outline XTWYTFMLZFPYCI-KQYNXXCUSA-K SMILEShelp_outline Nc1ncnc2n(cnc12)[C@@H]1O[C@H](COP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]1O 2D coordinates Mol file for the small molecule Search links Involved in 841 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline amicoumacin A 2-phosphate Identifier CHEBI:144859 Charge -1 Formula C20H29N3O10P InChIKeyhelp_outline HOJHDNFGPGIISV-YKRRISCLSA-M SMILEShelp_outline C1(=CC=CC2=C1C(O[C@@](C2)([C@@H](NC([C@H]([C@H]([C@H](CC(N)=O)[NH3+])O)OP(=O)([O-])[O-])=O)CC(C)C)[H])=O)O 2D coordinates Mol file for the small molecule Search links Involved in 2 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline H+ Identifier CHEBI:15378 Charge 1 Formula H InChIKeyhelp_outline GPRLSGONYQIRFK-UHFFFAOYSA-N SMILEShelp_outline [H+] 2D coordinates Mol file for the small molecule Search links Involved in 9,431 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Cross-references
| RHEA:61588 | RHEA:61589 | RHEA:61590 | RHEA:61591 | |
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| Reaction direction help_outline | undefined | left-to-right | right-to-left | bidirectional |
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Publications
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Ultrahigh-throughput functional profiling of microbiota communities.
Terekhov S.S., Smirnov I.V., Malakhova M.V., Samoilov A.E., Manolov A.I., Nazarov A.S., Danilov D.V., Dubiley S.A., Osterman I.A., Rubtsova M.P., Kostryukova E.S., Ziganshin R.H., Kornienko M.A., Vanyushkina A.A., Bukato O.N., Ilina E.N., Vlasov V.V., Severinov K.V., Gabibov A.G., Altman S.
Microbiome spectra serve as critical clues to elucidate the evolutionary biology pathways, potential pathologies, and even behavioral patterns of the host organisms. Furthermore, exotic sources of microbiota represent an unexplored niche to discover microbial secondary metabolites. However, establ ... >> More
Microbiome spectra serve as critical clues to elucidate the evolutionary biology pathways, potential pathologies, and even behavioral patterns of the host organisms. Furthermore, exotic sources of microbiota represent an unexplored niche to discover microbial secondary metabolites. However, establishing the bacterial functionality is complicated by an intricate web of interactions inside the microbiome. Here we apply an ultrahigh-throughput (uHT) microfluidic droplet platform for activity profiling of the entire oral microbial community of the Siberian bear to isolate <i>Bacillus</i> strains demonstrating antimicrobial activity against <i>Staphylococcus aureus</i> Genome mining allowed us to identify antibiotic amicoumacin A (Ami) as responsible for inhibiting the growth of <i>S. aureus</i> Proteomics and metabolomics revealed a unique mechanism of <i>Bacillus</i> self-resistance to Ami, based on a subtle equilibrium of its deactivation and activation by kinase AmiN and phosphatase AmiO, respectively. We developed uHT quantitative single-cell analysis to estimate antibiotic efficacy toward different microbiomes and used it to determine the activity spectra of Ami toward human and Siberian bear microbiota. Thus, uHT microfluidic droplet platform activity profiling is a powerful tool for discovering antibiotics and quantifying external influences on a microbiome. << Less
Proc. Natl. Acad. Sci. U.S.A. 115:9551-9556(2018) [PubMed] [EuropePMC]
This publication is cited by 1 other entry.