Reaction participants Show >> << Hide
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Namehelp_outline
adenosine4220 in 28S rRNA
Identifier
RHEA-COMP:16142
Reactive part
help_outline
- Name help_outline AMP residue Identifier CHEBI:74411 Charge -1 Formula C10H11N5O6P Positionhelp_outline 4220 SMILEShelp_outline NC1=NC=NC2=C1N=CN2[C@@H]3O[C@H](COP(=O)(*)[O-])[C@@H](O*)[C@H]3O 2D coordinates Mol file for the small molecule Search links Involved in 40 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline S-adenosyl-L-methionine Identifier CHEBI:59789 Charge 1 Formula C15H23N6O5S InChIKeyhelp_outline MEFKEPWMEQBLKI-AIRLBKTGSA-O SMILEShelp_outline C[S+](CC[C@H]([NH3+])C([O-])=O)C[C@H]1O[C@H]([C@H](O)[C@@H]1O)n1cnc2c(N)ncnc12 2D coordinates Mol file for the small molecule Search links Involved in 904 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
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Namehelp_outline
N6-methyladenosine4220 in 28S rRNA
Identifier
RHEA-COMP:16143
Reactive part
help_outline
- Name help_outline N6-methyladenosine 5'-phosphate residue Identifier CHEBI:74449 Charge -1 Formula C11H13N5O6P Positionhelp_outline 4220 SMILEShelp_outline C=1(NC)N=CN=C2C1N=CN2[C@@H]3O[C@H](COP(=O)(*)[O-])[C@@H](O*)[C@H]3O 2D coordinates Mol file for the small molecule Search links Involved in 11 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline S-adenosyl-L-homocysteine Identifier CHEBI:57856 Charge 0 Formula C14H20N6O5S InChIKeyhelp_outline ZJUKTBDSGOFHSH-WFMPWKQPSA-N SMILEShelp_outline Nc1ncnc2n(cnc12)[C@@H]1O[C@H](CSCC[C@H]([NH3+])C([O-])=O)[C@@H](O)[C@H]1O 2D coordinates Mol file for the small molecule Search links Involved in 827 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline H+ Identifier CHEBI:15378 Charge 1 Formula H InChIKeyhelp_outline GPRLSGONYQIRFK-UHFFFAOYSA-N SMILEShelp_outline [H+] 2D coordinates Mol file for the small molecule Search links Involved in 9,521 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Cross-references
| RHEA:58724 | RHEA:58725 | RHEA:58726 | RHEA:58727 | |
|---|---|---|---|---|
| Reaction direction help_outline | undefined | left-to-right | right-to-left | bidirectional |
| UniProtKB help_outline |
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Related reactions help_outline
More general form(s) of this reaction
Publications
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N6-methyladenosine methyltransferase ZCCHC4 mediates ribosomal RNA methylation.
Ma H., Wang X., Cai J., Dai Q., Natchiar S.K., Lv R., Chen K., Lu Z., Chen H., Shi Y.G., Lan F., Fan J., Klaholz B.P., Pan T., Shi Y., He C.
N<sup>6</sup>-Methyladenosine (m<sup>6</sup>A) RNA modification is present in messenger RNAs (mRNA), ribosomal RNAs (rRNA), and spliceosomal RNAs (snRNA) in humans. Although mRNA m<sup>6</sup>A modifications have been extensively studied and shown to play critical roles in many cellular processes, ... >> More
N<sup>6</sup>-Methyladenosine (m<sup>6</sup>A) RNA modification is present in messenger RNAs (mRNA), ribosomal RNAs (rRNA), and spliceosomal RNAs (snRNA) in humans. Although mRNA m<sup>6</sup>A modifications have been extensively studied and shown to play critical roles in many cellular processes, the identity of m<sup>6</sup>A methyltransferases for rRNAs and the function of rRNA m<sup>6</sup>A modifications are unknown. Here we report a new m<sup>6</sup>A methyltransferase, ZCCHC4, which primarily methylates human 28S rRNA and also interacts with a subset of mRNAs. ZCCHC4 knockout eliminates m<sup>6</sup>A4220 modification in 28S rRNA, reduces global translation, and inhibits cell proliferation. We also find that ZCCHC4 protein is overexpressed in hepatocellular carcinoma tumors, and ZCCHC4 knockout significantly reduces tumor size in a xenograft mouse model. Our results highlight the functional significance of an rRNA m<sup>6</sup>A modification in translation and in tumor biology. << Less