Enzymes
UniProtKB help_outline | 333 proteins |
Enzyme class help_outline |
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- Name help_outline Ni-sirohydrochlorin Identifier CHEBI:136841 Charge -8 Formula C42H36N4NiO16 InChIKeyhelp_outline CSCBRZIGJLRQGM-QIISWYHFSA-D SMILEShelp_outline C=12[C@H]([C@@](CC([O-])=O)(C)C=3[N+]1[Ni-2]45N6C(C3)=C(C(CCC([O-])=O)=C6C=C7[N+]5=C(C=C8N4C(=C2)[C@]([C@@H]8CCC([O-])=O)(CC([O-])=O)C)C(=C7CCC([O-])=O)CC([O-])=O)CC([O-])=O)CCC([O-])=O 2D coordinates Mol file for the small molecule Search links Involved in 2 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline H+ Identifier CHEBI:15378 Charge 1 Formula H InChIKeyhelp_outline GPRLSGONYQIRFK-UHFFFAOYSA-N SMILEShelp_outline [H+] 2D coordinates Mol file for the small molecule Search links Involved in 9,521 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline sirohydrochlorin Identifier CHEBI:58351 Charge -8 Formula C42H38N4O16 InChIKeyhelp_outline KWIZRXMMFRBUML-AHGFGAHVSA-F SMILEShelp_outline C[C@]1(CC([O-])=O)[C@H](CCC([O-])=O)c2cc3[nH]c(cc4nc(cc5[nH]c(cc1n2)c(CC([O-])=O)c5CCC([O-])=O)c(CCC([O-])=O)c4CC([O-])=O)[C@@H](CCC([O-])=O)[C@]3(C)CC([O-])=O 2D coordinates Mol file for the small molecule Search links Involved in 4 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline Ni2+ Identifier CHEBI:49786 (CAS: 14701-22-5) help_outline Charge 2 Formula Ni InChIKeyhelp_outline VEQPNABPJHWNSG-UHFFFAOYSA-N SMILEShelp_outline [Ni++] 2D coordinates Mol file for the small molecule Search links Involved in 5 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Cross-references
RHEA:52796 | RHEA:52797 | RHEA:52798 | RHEA:52799 | |
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Reaction direction help_outline | undefined | left-to-right | right-to-left | bidirectional |
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Publications
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The biosynthetic pathway of coenzyme F430 in methanogenic and methanotrophic archaea.
Zheng K., Ngo P.D., Owens V.L., Yang X.P., Mansoorabadi S.O.
Methyl-coenzyme M reductase (MCR) is the key enzyme of methanogenesis and anaerobic methane oxidation. The activity of MCR is dependent on the unique nickel-containing tetrapyrrole known as coenzyme F430. We used comparative genomics to identify the coenzyme F430 biosynthesis (cfb) genes and chara ... >> More
Methyl-coenzyme M reductase (MCR) is the key enzyme of methanogenesis and anaerobic methane oxidation. The activity of MCR is dependent on the unique nickel-containing tetrapyrrole known as coenzyme F430. We used comparative genomics to identify the coenzyme F430 biosynthesis (cfb) genes and characterized the encoded enzymes from Methanosarcina acetivorans C2A. The pathway involves nickelochelation by a nickel-specific chelatase, followed by amidation to form Ni-sirohydrochlorin a,c-diamide. Next, a primitive homolog of nitrogenase mediates a six-electron reduction and γ-lactamization reaction before a Mur ligase homolog forms the six-membered carbocyclic ring in the final step of the pathway. These data show that coenzyme F430 can be synthesized from sirohydrochlorin using Cfb enzymes produced heterologously in a nonmethanogen host and identify several targets for inhibitors of biological methane formation. << Less
Science 354:339-342(2016) [PubMed] [EuropePMC]
This publication is cited by 3 other entries.
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Elucidation of the biosynthesis of the methane catalyst coenzyme F430.
Moore S.J., Sowa S.T., Schuchardt C., Deery E., Lawrence A.D., Ramos J.V., Billig S., Birkemeyer C., Chivers P.T., Howard M.J., Rigby S.E., Layer G., Warren M.J.
Methane biogenesis in methanogens is mediated by methyl-coenzyme M reductase, an enzyme that is also responsible for the utilization of methane through anaerobic methane oxidation. The enzyme uses an ancillary factor called coenzyme F<sub>430</sub>, a nickel-containing modified tetrapyrrole that p ... >> More
Methane biogenesis in methanogens is mediated by methyl-coenzyme M reductase, an enzyme that is also responsible for the utilization of methane through anaerobic methane oxidation. The enzyme uses an ancillary factor called coenzyme F<sub>430</sub>, a nickel-containing modified tetrapyrrole that promotes catalysis through a methyl radical/Ni(ii)-thiolate intermediate. However, it is unclear how coenzyme F<sub>430</sub> is synthesized from the common primogenitor uroporphyrinogen iii, incorporating 11 steric centres into the macrocycle, although the pathway must involve chelation, amidation, macrocyclic ring reduction, lactamization and carbocyclic ring formation. Here we identify the proteins that catalyse the biosynthesis of coenzyme F<sub>430</sub> from sirohydrochlorin, termed CfbA-CfbE, and demonstrate their activity. The research completes our understanding of how the repertoire of tetrapyrrole-based pigments are constructed, permitting the development of recombinant systems to use these metalloprosthetic groups more widely. << Less
Nature 543:78-82(2017) [PubMed] [EuropePMC]
This publication is cited by 1 other entry.