Reaction participants Show >> << Hide
- Name help_outline dTMP Identifier CHEBI:63528 (Beilstein: 3916216) help_outline Charge -2 Formula C10H13N2O8P InChIKeyhelp_outline GYOZYWVXFNDGLU-XLPZGREQSA-L SMILEShelp_outline Cc1cn([C@H]2C[C@H](O)[C@@H](COP([O-])([O-])=O)O2)c(=O)[nH]c1=O 2D coordinates Mol file for the small molecule Search links Involved in 14 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline H2O Identifier CHEBI:15377 (Beilstein: 3587155; CAS: 7732-18-5) help_outline Charge 0 Formula H2O InChIKeyhelp_outline XLYOFNOQVPJJNP-UHFFFAOYSA-N SMILEShelp_outline [H]O[H] 2D coordinates Mol file for the small molecule Search links Involved in 6,204 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline 2-deoxy-D-ribose 5-phosphate Identifier CHEBI:62877 Charge -2 Formula C5H9O7P InChIKeyhelp_outline KKZFLSZAWCYPOC-PYHARJCCSA-L SMILEShelp_outline [C@H]1([C@H](CC(O1)O)O)COP([O-])(=O)[O-] 2D coordinates Mol file for the small molecule Search links Involved in 11 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline thymine Identifier CHEBI:17821 (Beilstein: 607626; CAS: 65-71-4) help_outline Charge 0 Formula C5H6N2O2 InChIKeyhelp_outline RWQNBRDOKXIBIV-UHFFFAOYSA-N SMILEShelp_outline Cc1c[nH]c(=O)[nH]c1=O 2D coordinates Mol file for the small molecule Search links Involved in 11 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Cross-references
| RHEA:52712 | RHEA:52713 | RHEA:52714 | RHEA:52715 | |
|---|---|---|---|---|
| Reaction direction help_outline | undefined | left-to-right | right-to-left | bidirectional |
| UniProtKB help_outline |
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Related reactions help_outline
More general form(s) of this reaction
Publications
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Nontargeted in vitro metabolomics for high-throughput identification of novel enzymes in Escherichia coli.
Sevin D.C., Fuhrer T., Zamboni N., Sauer U.
Our understanding of metabolism is limited by a lack of knowledge about the functions of many enzymes. Here, we develop a high-throughput mass spectrometry approach to comprehensively profile proteins for in vitro enzymatic activity. Overexpressed or purified proteins are incubated in a supplement ... >> More
Our understanding of metabolism is limited by a lack of knowledge about the functions of many enzymes. Here, we develop a high-throughput mass spectrometry approach to comprehensively profile proteins for in vitro enzymatic activity. Overexpressed or purified proteins are incubated in a supplemented metabolome extract containing hundreds of biologically relevant candidate substrates, and accumulating and depleting metabolites are determined by nontargeted mass spectrometry. By combining chemometrics and database approaches, we established an automated pipeline for unbiased annotation of the functions of novel enzymes. In screening all 1,275 functionally uncharacterized Escherichia coli proteins, we discovered 241 potential novel enzymes, 12 of which we experimentally validated. Our high-throughput in vitro metabolomics method is generally applicable to any purified protein or crude cell lysate of its overexpression host and enables performing up to 1,200 nontargeted enzyme assays per working day. << Less
Nat. Methods 14:187-194(2017) [PubMed] [EuropePMC]
This publication is cited by 30 other entries.