Enzymes
| UniProtKB help_outline | 1 proteins |
| Enzyme class help_outline |
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Name help_outline
an archaeal dolichyl N-acetyl-α-D-glucosaminyl phosphate
Identifier
CHEBI:88021
Charge
-1
Formula
(C5H8)n.C28H51NO9P
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Involved in 1 reaction(s)
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Form(s) in this reaction:
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Identifier: RHEA-COMP:11931Polymer name: an archaeal dolichyl N-acetyl-α-D-glucosaminyl phosphatePolymerization index help_outline nFormula C28H51NO9P(C5H8)nCharge (-1)(0)nMol File for the polymer
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- Name help_outline UDP-2,3-diacetamido-2,3-dideoxy-α-D-glucuronate Identifier CHEBI:58745 (Beilstein: 11962414) help_outline Charge -3 Formula C19H25N4O18P2 InChIKeyhelp_outline GZLIMKLKXDFTJR-LTMKHLKMSA-K SMILEShelp_outline CC(=O)N[C@H]1[C@H](O)[C@H](O[C@H](OP([O-])(=O)OP([O-])(=O)OC[C@H]2O[C@H]([C@H](O)[C@@H]2O)n2ccc(=O)[nH]c2=O)[C@@H]1NC(C)=O)C([O-])=O 2D coordinates Mol file for the small molecule Search links Involved in 3 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
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Name help_outline
an archaeal dolichyl 3-O-(2,3-diacetamido-2,3-dideoxy- β-D-glucuronsyl)-N-acetyl- α-D-glucosaminyl phosphate
Identifier
CHEBI:88026
Charge
-2
Formula
(C5H8)n.C38H64N3O15P
Search links
Involved in 1 reaction(s)
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Form(s) in this reaction:
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Identifier: RHEA-COMP:12047Polymer name: an archaeal dolichyl 3-O-(2,3-diacetamido-2,3-dideoxy- β-D-glucuronosyl)-N-acetyl- α-D-glucosaminyl phosphatePolymerization index help_outline nFormula C38H64N3O15P(C5H8)nCharge (-2)(0)nMol File for the polymer
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- Name help_outline UDP Identifier CHEBI:58223 Charge -3 Formula C9H11N2O12P2 InChIKeyhelp_outline XCCTYIAWTASOJW-XVFCMESISA-K SMILEShelp_outline O[C@@H]1[C@@H](COP([O-])(=O)OP([O-])([O-])=O)O[C@H]([C@@H]1O)n1ccc(=O)[nH]c1=O 2D coordinates Mol file for the small molecule Search links Involved in 577 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline H+ Identifier CHEBI:15378 Charge 1 Formula H InChIKeyhelp_outline GPRLSGONYQIRFK-UHFFFAOYSA-N SMILEShelp_outline [H+] 2D coordinates Mol file for the small molecule Search links Involved in 9,521 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Cross-references
| RHEA:47596 | RHEA:47597 | RHEA:47598 | RHEA:47599 | |
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| Reaction direction help_outline | undefined | left-to-right | right-to-left | bidirectional |
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Publications
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Biochemical evidence for an alternate pathway in N-linked glycoprotein biosynthesis.
Larkin A., Chang M.M., Whitworth G.E., Imperiali B.
Asparagine-linked glycosylation is a complex protein modification conserved among all three domains of life. Herein we report the in vitro analysis of N-linked glycosylation from the methanogenic archaeon Methanococcus voltae. Using a suite of synthetic and semisynthetic substrates, we show that A ... >> More
Asparagine-linked glycosylation is a complex protein modification conserved among all three domains of life. Herein we report the in vitro analysis of N-linked glycosylation from the methanogenic archaeon Methanococcus voltae. Using a suite of synthetic and semisynthetic substrates, we show that AglK initiates N-linked glycosylation in M. voltae through the formation of α-linked dolichyl monophosphate N-acetylglucosamine, which contrasts with the polyprenyl diphosphate intermediates that feature in both eukaryotes and bacteria. Notably, AglK has high sequence homology to dolichyl phosphate β-glucosyltransferases, including Alg5 in eukaryotes, suggesting a common evolutionary origin. The combined action of the first two enzymes, AglK and AglC, afforded an α-linked dolichyl monophosphate glycan that serves as a competent substrate for the archaeal oligosaccharyl transferase AglB. These studies provide what is to our knowledge the first biochemical evidence revealing that, despite the apparent similarity of the overall pathways, there are actually two general strategies to achieve N-linked glycoproteins across the domains of life. << Less
Nat. Chem. Biol. 9:367-373(2013) [PubMed] [EuropePMC]
This publication is cited by 1 other entry.
Comments
Dolichol used by archaea is different from that used by eukaryotes. It is much shorter (C55-C60), it is α,ω-saturated and it may have additional unsaturated positions in the chain.