Rhea - reaction knowledgebase

Reaction participants Show >> << Hide

Name ^help_outline CoA Identifier CHEBI:57287 (Beilstein: 11604429) ^help_outline Charge -4 Formula C21H32N7O16P3S InChIKey^help_outline RGJOEKWQDUBAIZ-IBOSZNHHSA-J SMILES^help_outline CC(C)(COP([O-])(=O)OP([O-])(=O)OC[C@H]1O[C@H]([C@H](O)[C@@H]1OP([O-])([O-])=O)n1cnc2c(N)ncnc12)[C@@H](O)C(=O)NCCC(=O)NCCS 2D coordinates Mol file for the small molecule Search links Involved in 1,500 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Name ^help_outline H⁺ Identifier CHEBI:15378 Charge 1 Formula H InChIKey^help_outline GPRLSGONYQIRFK-UHFFFAOYSA-N SMILES^help_outline [H+] 2D coordinates Mol file for the small molecule Search links Involved in 9,431 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Name^help_outline oxidized [flavodoxin] Identifier RHEA-COMP:10623 Reactive part ^help_outline
- Name ^help_outline FMN Identifier CHEBI:58210 Charge -3 Formula C17H18N4O9P InChIKey^help_outline ANKZYBDXHMZBDK-SCRDCRAPSA-K SMILES^help_outline C12=NC([N-]C(C1=NC=3C(N2C[C@@H]([C@@H]([C@@H](COP(=O)([O-])[O-])O)O)O)=CC(=C(C3)C)C)=O)=O 2D coordinates Mol file for the small molecule Search links Involved in 804 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Search links Involved in 17 reaction(s) Find proteins in UniProtKB for this molecule
Name ^help_outline pyruvate Identifier CHEBI:15361 (Beilstein: 3587721; CAS: 57-60-3) ^help_outline Charge -1 Formula C3H3O3 InChIKey^help_outline LCTONWCANYUPML-UHFFFAOYSA-M SMILES^help_outline CC(=O)C([O-])=O 2D coordinates Mol file for the small molecule Search links Involved in 215 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Name ^help_outline acetyl-CoA Identifier CHEBI:57288 (Beilstein: 8468140) ^help_outline Charge -4 Formula C23H34N7O17P3S InChIKey^help_outline ZSLZBFCDCINBPY-ZSJPKINUSA-J SMILES^help_outline CC(=O)SCCNC(=O)CCNC(=O)[C@H](O)C(C)(C)COP([O-])(=O)OP([O-])(=O)OC[C@H]1O[C@H]([C@H](O)[C@@H]1OP([O-])([O-])=O)n1cnc2c(N)ncnc12 2D coordinates Mol file for the small molecule Search links Involved in 352 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Name ^help_outline CO₂ Identifier CHEBI:16526 (Beilstein: 1900390; CAS: 124-38-9) ^help_outline Charge 0 Formula CO2 InChIKey^help_outline CURLTUGMZLYLDI-UHFFFAOYSA-N SMILES^help_outline O=C=O 2D coordinates Mol file for the small molecule Search links Involved in 997 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Name^help_outline reduced [flavodoxin] Identifier RHEA-COMP:10622 Reactive part ^help_outline
- Name ^help_outline FMNH₂ Identifier CHEBI:57618 (Beilstein: 6258176) ^help_outline Charge -2 Formula C17H21N4O9P InChIKey^help_outline YTNIXZGTHTVJBW-SCRDCRAPSA-L SMILES^help_outline Cc1cc2Nc3c([nH]c(=O)[nH]c3=O)N(C[C@H](O)[C@H](O)[C@H](O)COP([O-])([O-])=O)c2cc1C 2D coordinates Mol file for the small molecule Search links Involved in 794 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Search links Involved in 19 reaction(s) Find proteins in UniProtKB for this molecule

Cross-references

	RHEA:44140	RHEA:44141	RHEA:44142	RHEA:44143
Reaction direction	undefined	left-to-right	right-to-left	bidirectional
UniProtKB ^help_outline	2,863 proteins
Gene Ontology ^help_outline	GO:0043873
KEGG ^help_outline				R01196
MetaCyc ^help_outline				PYFLAVOXRE-RXN
EcoCyc ^help_outline				PYFLAVOXRE-RXN

Publications

Electron transport to nitrogenase. Purification and characterization of pyruvate:flavodoxin oxidoreductase. The nifJ gene product.

Shah V.K., Stacey G., Brill W.J.

Pyruvate:flavodoxin oxidoreductase, the nifJ gene product of Klebsiella pneumoniae, was purified to homogeneity. Pyruvate:flavodoxin oxidoreductase, flavodoxin, and nitrogenase components I and II are the only proteins required for pyruvate-coupled nitrogenase activity. The physiological source of ... >> More

Pyruvate:flavodoxin oxidoreductase, the nifJ gene product of Klebsiella pneumoniae, was purified to homogeneity. Pyruvate:flavodoxin oxidoreductase, flavodoxin, and nitrogenase components I and II are the only proteins required for pyruvate-coupled nitrogenase activity. The physiological source of electrons to nitrogenase in K. pneumoniae is pyruvate. Flavodoxin from Azotobacter vinelandii was only one-third as effective as K. pneumoniae flavodoxin in transferring electrons from pyruvate:flavodoxin oxidoreductase to Azotobacter and Klebsiella nitrogenases. Ferredoxins from aerobic, anaerobic and photosynthetic nitrogen-fixing organisms, as well as benzyl viologen and methyl viologen, were ineffective in coupling pyruvate oxidation to nitrogenase activity. One mol each of acetyl-CoA, CO2, and ethylene are formed by pyruvate-supported acetylene reduction. The enzyme contains 8.0 +/-0.6 mol of iron and 6.6 +/-0.2 mol of acid-labile sulfide per mol of protein (Mr = 240,000). Pyruvate:flavodoxin oxidoreductase is irreversibly inactivated by air. << Less

J Biol Chem 258:12064-12068(1983) [PubMed] [EuropePMC]

RHEA:44143 CoA + 2 H(+) + oxidized [flavodoxin] + pyruvate <=> acetyl-CoA + CO2 + reduced [flavodoxin] Reaction with equal flow from both directions. help_outline

Reaction participants Show >> << Hide

Cross-references

Publications

Electron transport to nitrogenase. Purification and characterization of pyruvate:flavodoxin oxidoreductase. The nifJ gene product.

RHEA:44143 CoA + 2 H(+) + oxidized [flavodoxin] + pyruvate <=> acetyl-CoA + CO2 + reduced [flavodoxin] Reaction with equal flow from both directions. ^help_outline