Enzymes
UniProtKB help_outline | 1 proteins |
Enzyme class help_outline |
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Reaction participants Show >> << Hide
- Name help_outline all-trans-8'-apo-β-carotenal Identifier CHEBI:53154 (Beilstein: 2064131; CAS: 1107-26-2) help_outline Charge 0 Formula C30H40O InChIKeyhelp_outline DFMMVLFMMAQXHZ-DOKBYWHISA-N SMILEShelp_outline [H]C(=O)C(\C)=C\C=C\C(C)=C\C=C\C=C(C)\C=C\C=C(C)\C=C\C1=C(C)CCCC1(C)C 2D coordinates Mol file for the small molecule Search links Involved in 2 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline O2 Identifier CHEBI:15379 (CAS: 7782-44-7) help_outline Charge 0 Formula O2 InChIKeyhelp_outline MYMOFIZGZYHOMD-UHFFFAOYSA-N SMILEShelp_outline O=O 2D coordinates Mol file for the small molecule Search links Involved in 2,709 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline 13-apo-β-carotenone Identifier CHEBI:53175 (Beilstein: 2052829; CAS: 85354-07-0) help_outline Charge 0 Formula C18H26O InChIKeyhelp_outline UBTNVRPIHJRBCI-LUXGDSJYSA-N SMILEShelp_outline CC(=O)\C=C\C=C(C)\C=C\C1=C(C)CCCC1(C)C 2D coordinates Mol file for the small molecule Search links Involved in 2 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline 2,6-dimethyldeca-2,4,6,8-tetraenedial Identifier CHEBI:73152 Charge 0 Formula C12H14O2 InChIKeyhelp_outline NOURWHKLHSWPGX-KIUBFKPTSA-N SMILEShelp_outline [H]C(=O)\C=C\C=C(C)\C=C\C=C(/C)C([H])=O 2D coordinates Mol file for the small molecule Search links Involved in 1 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Cross-references
RHEA:36043 | RHEA:36044 | RHEA:36045 | RHEA:36046 | |
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Reaction direction help_outline | undefined | left-to-right | right-to-left | bidirectional |
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Publications
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Characterization of an apo-carotenoid 13,14-dioxygenase from Novosphingobium aromaticivorans that converts beta-apo-8'-carotenal to beta-apo-13-carotenone.
Kim Y.S., Seo E.S., Oh D.K.
A putative carotenoid oxygenase from Novosphingobium aromaticivorans was purified with a specific activity of 0.8 U/mg by His-Trap affinity chromatography. The native enzyme was estimated to be a 52 kDa monomer. Enzyme activity for β-apo-8'-carotenal was maximal at pH 8.0 and 45 °C, with a half li ... >> More
A putative carotenoid oxygenase from Novosphingobium aromaticivorans was purified with a specific activity of 0.8 U/mg by His-Trap affinity chromatography. The native enzyme was estimated to be a 52 kDa monomer. Enzyme activity for β-apo-8'-carotenal was maximal at pH 8.0 and 45 °C, with a half life of 15.3 h, K(m) of 21 μM, and k(cat) of 25 l/min. The enzyme exhibited cleavage activity only for carotenoids containing one β-ionone ring and its catalytic efficiency (k(cat)/K(m)) followed the order β-apo-8'-carotenal > β-apo-4'-carotenal > γ-carotene. The enzyme converted these carotenoids to β-apo-13-carotenones by cleaving their C(13)-C(14) double bonds. The oxygen atom of β-apo-13-carotenone originated not from water but from molecular oxygen. Thus, the enzyme was an apo-carotenoid 13,14-dioxygenase. << Less