Enzymes
| UniProtKB help_outline | 4 proteins |
| Enzyme class help_outline |
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- Name help_outline scyllo-inosose Identifier CHEBI:17811 (CAS: 488-64-2) help_outline Charge 0 Formula C6H10O6 InChIKeyhelp_outline VYEGBDHSGHXOGT-HYFGLKJPSA-N SMILEShelp_outline O[C@H]1[C@H](O)[C@@H](O)C(=O)[C@@H](O)[C@@H]1O 2D coordinates Mol file for the small molecule Search links Involved in 8 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline scyllo-inosine Identifier CHEBI:50920 (Beilstein: 2692511) help_outline Charge 0 Formula C6H10O6 InChIKeyhelp_outline VYEGBDHSGHXOGT-QFYCRYKCSA-N SMILEShelp_outline O[C@H]1[C@H](O)[C@@H](O)C(=O)[C@H](O)[C@@H]1O 2D coordinates Mol file for the small molecule Search links Involved in 2 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Cross-references
| RHEA:25776 | RHEA:25777 | RHEA:25778 | RHEA:25779 | |
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| Reaction direction help_outline | undefined | left-to-right | right-to-left | bidirectional |
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Publications
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Genetic modification of Bacillus subtilis for production of D-chiro-inositol, an investigational drug candidate for treatment of type 2 diabetes and polycystic ovary syndrome.
Yoshida K., Yamaguchi M., Morinaga T., Ikeuchi M., Kinehara M., Ashida H.
D-chiro-inositol (DCI) is a drug candidate for the treatment of type 2 diabetes and polycystic ovary syndrome, since it improves the efficiency with which the body uses insulin and also promotes ovulation. Here, we report genetic modification of Bacillus subtilis for production of DCI from myo-ino ... >> More
D-chiro-inositol (DCI) is a drug candidate for the treatment of type 2 diabetes and polycystic ovary syndrome, since it improves the efficiency with which the body uses insulin and also promotes ovulation. Here, we report genetic modification of Bacillus subtilis for production of DCI from myo-inositol (MI). The B. subtilis iolABCDEFGHIJ operon encodes enzymes for the multiple steps of the MI catabolic pathway. In the first and second steps, MI is converted to 2-keto-MI (2KMI) by IolG and then to 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione by IolE. In this study, we identified iolI encoding inosose isomerase, which converts 2KMI to 1-keto-D-chiro-inositol (1KDCI), and found that IolG reduces 1KDCI to DCI. Inactivation of iolE in a mutant constitutively expressing the iol operon blocked the MI catabolic pathway to accumulate 2KMI, which was converted to DCI via the activity of IolI and IolG. The mutant was able to convert at least 6% of input MI in the culture medium to DCI. << Less
Appl. Environ. Microbiol. 72:1310-1315(2006) [PubMed] [EuropePMC]
This publication is cited by 1 other entry.
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Crystal structure of Bacillus subtilis iolI shows endonuclease IV fold with altered Zn binding.
Zhang R.G., Dementieva I., Duke N., Collart F., Quaite-Randall E., Alkire R., Dieckman L., Maltsev N., Korolev O., Joachimiak A.