Enzymes
| UniProtKB help_outline | 1 proteins |
| Enzyme class help_outline |
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Reaction participants Show >> << Hide
- Name help_outline A Identifier CHEBI:13193 Charge Formula R SMILEShelp_outline * 2D coordinates Mol file for the small molecule Search links Involved in 2,870 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline L-sorbopyranose Identifier CHEBI:48649 (Beilstein: 2689728) help_outline Charge 0 Formula C6H12O6 InChIKeyhelp_outline LKDRXBCSQODPBY-AMVSKUEXSA-N SMILEShelp_outline OCC1(O)OC[C@H](O)[C@@H](O)[C@@H]1O 2D coordinates Mol file for the small molecule Search links Involved in 1 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline 1-dehydro-L-sorbose Identifier CHEBI:48657 (Beilstein: 1725426) help_outline Charge 0 Formula C6H10O6 InChIKeyhelp_outline DCNMIDLYWOTSGK-KVQBGUIXSA-N SMILEShelp_outline [H]C(=O)C(=O)[C@@H](O)[C@H](O)[C@@H](O)CO 2D coordinates Mol file for the small molecule Search links Involved in 1 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline AH2 Identifier CHEBI:17499 Charge 0 Formula RH2 SMILEShelp_outline *([H])[H] 2D coordinates Mol file for the small molecule Search links Involved in 2,799 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Cross-references
| RHEA:24878 | RHEA:24879 | RHEA:24880 | RHEA:24881 | |
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| Reaction direction help_outline | undefined | left-to-right | right-to-left | bidirectional |
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Publications
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Isolation and Characterization of a New Quinoprotein Dehydrogenase, L-Sorbose/L-Sorbosone Dehydrogenase.
Asakura A., Hoshino T.
Gluconobacter oxydans DSM 4025 effectively oxidizes L-sorbose to 2-keto-L-gulonic acid (2 KGA), an industrial precursor of vitamin C. From this microorganism, we purified the enzyme involved in this oxidation reaction. The enzyme is a unique quinoprotein dehydrogenase catalyzing not only the conve ... >> More
Gluconobacter oxydans DSM 4025 effectively oxidizes L-sorbose to 2-keto-L-gulonic acid (2 KGA), an industrial precursor of vitamin C. From this microorganism, we purified the enzyme involved in this oxidation reaction. The enzyme is a unique quinoprotein dehydrogenase catalyzing not only the conversion of L-sorbose to L-sorbosone, but also that of L-sorbosone to 2 KGA. The molecular weight of the enzyme was about 135,000, consisting of two subunits with molecular weights of 64,500 and 62,500. As its prosthetic group, non-covalently bound PQQ was found. The dye-linked spectrophotometric enzyme assay showed that the optimum enzyme activity occurred in the pH range about 7.0-9.0, and the enzyme activity was inhibited by EDTA or EGTA. The enzyme showed extremely broad substrate specificity for primary and secondary alcohols, aldehydes, aldoses, ketoses, and other sugar alcohols, but not for methanol or formaldehyde. The cytochrome c obtained from the soluble fraction of this strain was found to act as a physiological electron acceptor of the enzyme. << Less
Biosci Biotechnol Biochem 63:46-53(1999) [PubMed] [EuropePMC]
Comments
Published in: "Isolation and characterization of membrane-bound L-sorbose dehydrogenase from Gluconobacter melanogenus UV10." Sugisawa, T., Hoshino, T., Nomura, S. and Fujiwara, A. Agric. Biol. Chem. 55 (1991) 363–370.