Enzymes
| UniProtKB help_outline | 1 proteins |
| Enzyme class help_outline |
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| GO Molecular Function help_outline |
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- Name help_outline Cypridina luciferin Identifier CHEBI:58006 Charge 1 Formula C22H28N7O InChIKeyhelp_outline ZWPWSXGBDMGKKS-ZDUSSCGKSA-O SMILEShelp_outline [H][C@](C)(CC)c1nc2c(CCCNC(N)=[NH2+])[nH]c(cn2c1=O)-c1c[nH]c2ccccc12 2D coordinates Mol file for the small molecule Search links Involved in 1 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline O2 Identifier CHEBI:15379 (CAS: 7782-44-7) help_outline Charge 0 Formula O2 InChIKeyhelp_outline MYMOFIZGZYHOMD-UHFFFAOYSA-N SMILEShelp_outline O=O 2D coordinates Mol file for the small molecule Search links Involved in 2,727 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline oxidized Cypridina luciferin Identifier CHEBI:58059 Charge 1 Formula C21H28N7O InChIKeyhelp_outline PSYJEEMZZIZTSR-ZDUSSCGKSA-O SMILEShelp_outline CC[C@H](C)C(=O)Nc1ncc(nc1CCCNC(N)=[NH2+])-c1c[nH]c2ccccc12 2D coordinates Mol file for the small molecule Search links Involved in 1 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline hν Identifier CHEBI:30212 Charge 0 Formula SMILEShelp_outline * 2D coordinates Mol file for the small molecule Search links Involved in 25 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline CO2 Identifier CHEBI:16526 (CAS: 124-38-9) help_outline Charge 0 Formula CO2 InChIKeyhelp_outline CURLTUGMZLYLDI-UHFFFAOYSA-N SMILEShelp_outline O=C=O 2D coordinates Mol file for the small molecule Search links Involved in 1,006 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Cross-references
| RHEA:22760 | RHEA:22761 | RHEA:22762 | RHEA:22763 | |
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| Reaction direction help_outline | undefined | left-to-right | right-to-left | bidirectional |
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Publications
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Cloning and expression of cDNA for the luciferase from the marine ostracod Vargula hilgendorfii.
Thompson E.M., Nagata S., Tsuji F.I.
The marine ostracod Vargula hilgendorfii ejects luciferin and luciferase into seawater to produce a bright luminous cloud. The light is due to the oxidation of luciferin, an imidazopyrazine compound, by molecular oxygen, catalyzed by luciferase. The mechanism of the reaction has been studied exten ... >> More
The marine ostracod Vargula hilgendorfii ejects luciferin and luciferase into seawater to produce a bright luminous cloud. The light is due to the oxidation of luciferin, an imidazopyrazine compound, by molecular oxygen, catalyzed by luciferase. The mechanism of the reaction has been studied extensively and the 60 kcal/mol required for the blue emission have been shown to be derived from the oxidation of luciferin via a dioxetanone intermediate, in which the excited state oxyluciferin bound to luciferase is the emitter. However, only limited information is available regarding the properties of the enzyme. This paper reports the cloning and sequence analysis of the cDNA for Vargula luciferase and the expression of the cDNA in a mammalian cell system. The primary structure, deduced from the nucleotide sequence, consists of 555 amino acid residues in a single polypeptide chain with a molecular weight of 62,171. Two regions of the enzyme show significant amino acid sequence homology with an N-terminal segment of the photoprotein aequorin. The Vargula luciferase gene, which contains a signal sequence for secretion, should be well suited as a reporter in studies of gene expression. << Less
Proc. Natl. Acad. Sci. U.S.A. 86:6567-6571(1989) [PubMed] [EuropePMC]
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Mechanism of the enzyme--catalyzed oxidation of Cypridina and firefly luciferins studied by means of 17O2 and H218O1.
Tsuji F.I., DeLuca M., Boyer P.D., Endo S., Akutagawa M.
Biochem Biophys Res Commun 74:606-613(1977) [PubMed] [EuropePMC]
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Some properties of luciferase from the bioluminescent crustacean, Cypridina hilgendorfii.
Tsuji F.I., Lynch R.V., Stevens C.L.