Enzymes
UniProtKB help_outline | 6 proteins |
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Reaction participants Show >> << Hide
- Name help_outline D-mannose Identifier CHEBI:4208 (CAS: 530-26-7,3458-28-4,31103-86-3) help_outline Charge 0 Formula C6H12O6 InChIKeyhelp_outline WQZGKKKJIJFFOK-QTVWNMPRSA-N SMILEShelp_outline OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O 2D coordinates Mol file for the small molecule Search links Involved in 31 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline D-fructose Identifier CHEBI:37721 (Beilstein: 1680728; CAS: 57-48-7) help_outline Charge 0 Formula C6H12O6 InChIKeyhelp_outline RFSUNEUAIZKAJO-VRPWFDPXSA-N SMILEShelp_outline OC[C@H]1OC(O)(CO)[C@@H](O)[C@@H]1O 2D coordinates Mol file for the small molecule Search links Involved in 26 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Cross-references
RHEA:22604 | RHEA:22605 | RHEA:22606 | RHEA:22607 | |
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Reaction direction help_outline | undefined | left-to-right | right-to-left | bidirectional |
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Publications
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Production of d-mannose from d-glucose by co-expression of d-glucose isomerase and d-lyxose isomerase in Escherichia coli.
Huang J., Yu L., Zhang W., Zhang T., Guang C., Mu W.
<h4>Background</h4>d-Mannose is not only the epimer of d-glucose at the C-2 position, but also the aldose isomer of d-fructose. Because of its physiological properties and health benefits, d-mannose has attracted public interest. It has been confirmed that d-mannose has broad applications in food, ... >> More
<h4>Background</h4>d-Mannose is not only the epimer of d-glucose at the C-2 position, but also the aldose isomer of d-fructose. Because of its physiological properties and health benefits, d-mannose has attracted public interest. It has been confirmed that d-mannose has broad applications in food, cosmetics, and pharmaceutical industries. According to the Izumoring strategy, d-glucose isomerase (d-GI) and d-lyxose isomerase (d-LI) play important roles in the conversions of d-fructose from d-glucose and of d-mannose from d-fructose respectively. In this study, a one-step enzyme process of d-mannose production from d-glucose has been constructed by co-expression of the d-GI from Acidothermus cellulolyticus and d-LI from Thermosediminibacter oceani in Escherichia coli BL21(DE3) cells.<h4>Results</h4>The co-expression system exhibits maximum activity at pH 6.5 and 65 °C with Co<sup>2+</sup> supplement. It is relatively thermostable at less than 65 °C. When the reaction reaches equilibrium, the ratio of d-glucose, d-fructose, and d-mannose is approximately 34 : 49.6 : 16.4. By using this co-expression system, about 60.0 g L<sup>-1</sup> d-mannose is obtained from 400 g L<sup>-1</sup> d-glucose in 8 h.<h4>Conclusion</h4>This co-expression of d-GI and d-LI system provides a novel and efficient approach for d-mannose production. © 2018 Society of Chemical Industry. << Less
J Sci Food Agric 98:4895-4902(2018) [PubMed] [EuropePMC]
This publication is cited by 1 other entry.
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Mannose isomerase of Pseudomonas saccharophila.
PALLERONI N.J., DOUDOROFF M.