Reaction participants Show >> << Hide
- Name help_outline 1,2-bis(4-hydroxy-3-methoxyphenyl)ethylene Identifier CHEBI:17501 (Beilstein: 2057036) help_outline Charge 0 Formula C16H16O4 InChIKeyhelp_outline KQPXJFAYGYIGRU-UHFFFAOYSA-N SMILEShelp_outline [H]C(=C([H])c1ccc(O)c(OC)c1)c1ccc(O)c(OC)c1 2D coordinates Mol file for the small molecule Search links Involved in 1 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline O2 Identifier CHEBI:15379 (CAS: 7782-44-7) help_outline Charge 0 Formula O2 InChIKeyhelp_outline MYMOFIZGZYHOMD-UHFFFAOYSA-N SMILEShelp_outline O=O 2D coordinates Mol file for the small molecule Search links Involved in 2,709 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline vanillin Identifier CHEBI:18346 (Beilstein: 472792; CAS: 121-33-5) help_outline Charge 0 Formula C8H8O3 InChIKeyhelp_outline MWOOGOJBHIARFG-UHFFFAOYSA-N SMILEShelp_outline [H]C(=O)C1=CC(OC)=C(O)C=C1 2D coordinates Mol file for the small molecule Search links Involved in 14 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Cross-references
| RHEA:21340 | RHEA:21341 | RHEA:21342 | RHEA:21343 | |
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| Reaction direction help_outline | undefined | left-to-right | right-to-left | bidirectional |
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Publications
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Structural and enzymatical comparison of lignostilbene-alpha,beta-dioxygenase isozymes, I, II, and III, from Pseudomonas paucimobilis TMY1009.
Kamoda S., Saburi Y.
Three isozymes of lignostilbene-alpha,beta-dioxygenase (LSD) from Pseudomonas paucimobilis TMY1009 were separated on QAE-Toyopearl chromatography. All active fractions were further chromatographed on DEAE-Toyopearl, Butyl-Toyopearl, and Sephacryl S-300 columns. Then the isozymes I, II, and III wer ... >> More
Three isozymes of lignostilbene-alpha,beta-dioxygenase (LSD) from Pseudomonas paucimobilis TMY1009 were separated on QAE-Toyopearl chromatography. All active fractions were further chromatographed on DEAE-Toyopearl, Butyl-Toyopearl, and Sephacryl S-300 columns. Then the isozymes I, II, and III were purified homogeneously. All three isozymes consisted of two subunits with the same mol. mass. According to the N-terminal amino acid sequences up to 25 residues of these three isozymes and the reversed-phase HPLC patterns of peptidase-digested them, it was found that LSD-I, II, and III consisted of alpha alpha, alpha beta, and beta beta subunits, respectively. They showed different specificities for several substrates that are stilbene and styrene derivatives. << Less
Biosci. Biotechnol. Biochem. 57:931-934(1993) [PubMed] [EuropePMC]
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Cloning, expression, and sequence analysis of a lignostilbene-alpha,beta-dioxygenase gene from Pseudomonas paucimobilis TMY1009.
Kamoda S., Saburi Y.
From the genomic library of Pseudomonas paucimobilis TMY1009 constructed with the cosmid pHC79, an 8-kb BamHI-KpnI fragment encoding lignostilbene-alpha,beta-dioxygenase (LSD) was cloned into pUC19 (designated pKSN2510). E. coli JM109 having pKSN2510 produced a small amount of LSD only when the la ... >> More
From the genomic library of Pseudomonas paucimobilis TMY1009 constructed with the cosmid pHC79, an 8-kb BamHI-KpnI fragment encoding lignostilbene-alpha,beta-dioxygenase (LSD) was cloned into pUC19 (designated pKSN2510). E. coli JM109 having pKSN2510 produced a small amount of LSD only when the lac promoter was induced by isopropyl-beta-D-thio-galactopyranoside (IPTG). The 1.9-kb SalI fragment containing the LSD gene on pKSN2510 was subcloned into pUC119 in opposite directions (designated pKHN2560 and pKHN2590). The LSD gene on pKHN2590 was expressed in E. coli MV1184 using the lac promoter. LSD produced by E. coli MV1184 transformed with pKHN2590 (cloned LSD) was purified and found to be identical with LSD-I, which was the major isozyme produced by P. paucimobilis TMY1009. Cloned 1.9-kb nucleotide was sequenced and one open reading frame composed to 486 codons was found. << Less
Biosci. Biotechnol. Biochem. 57:926-930(1993) [PubMed] [EuropePMC]
Comments
Published in: "Purification and some properties of lignostilbene-alpha,beta-dioxygenase responsible for the c-alpha-c-beta cleavage of a diarylpropane type lignin model compound from Pseudomonas sp. TMY1009." Kamoda S., Habu N., Samejima M., Yoshimoto T. Agric. Biol. Chem. 53:2757-2761(1989)