Enzymes
| UniProtKB help_outline | 2 proteins |
| Enzyme class help_outline |
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| GO Molecular Function help_outline |
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- Name help_outline 2,4-dichlorophenol Identifier CHEBI:16738 (CAS: 120-83-2) help_outline Charge 0 Formula C6H4Cl2O InChIKeyhelp_outline HFZWRUODUSTPEG-UHFFFAOYSA-N SMILEShelp_outline Oc1ccc(Cl)cc1Cl 2D coordinates Mol file for the small molecule Search links Involved in 4 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline NADPH Identifier CHEBI:57783 (Beilstein: 10411862) help_outline Charge -4 Formula C21H26N7O17P3 InChIKeyhelp_outline ACFIXJIJDZMPPO-NNYOXOHSSA-J SMILEShelp_outline NC(=O)C1=CN(C=CC1)[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OC[C@H]2O[C@H]([C@H](OP([O-])([O-])=O)[C@@H]2O)n2cnc3c(N)ncnc23)[C@@H](O)[C@H]1O 2D coordinates Mol file for the small molecule Search links Involved in 1,288 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline O2 Identifier CHEBI:15379 (CAS: 7782-44-7) help_outline Charge 0 Formula O2 InChIKeyhelp_outline MYMOFIZGZYHOMD-UHFFFAOYSA-N SMILEShelp_outline O=O 2D coordinates Mol file for the small molecule Search links Involved in 2,727 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline H+ Identifier CHEBI:15378 Charge 1 Formula H InChIKeyhelp_outline GPRLSGONYQIRFK-UHFFFAOYSA-N SMILEShelp_outline [H+] 2D coordinates Mol file for the small molecule Search links Involved in 9,521 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline 3,5-dichlorocatechol Identifier CHEBI:15788 (CAS: 13673-92-2) help_outline Charge 0 Formula C6H4Cl2O2 InChIKeyhelp_outline XSXYVLIPQMXCBV-UHFFFAOYSA-N SMILEShelp_outline Oc1cc(Cl)cc(Cl)c1O 2D coordinates Mol file for the small molecule Search links Involved in 2 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline NADP+ Identifier CHEBI:58349 Charge -3 Formula C21H25N7O17P3 InChIKeyhelp_outline XJLXINKUBYWONI-NNYOXOHSSA-K SMILEShelp_outline NC(=O)c1ccc[n+](c1)[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OC[C@H]2O[C@H]([C@H](OP([O-])([O-])=O)[C@@H]2O)n2cnc3c(N)ncnc23)[C@@H](O)[C@H]1O 2D coordinates Mol file for the small molecule Search links Involved in 1,294 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline H2O Identifier CHEBI:15377 (CAS: 7732-18-5) help_outline Charge 0 Formula H2O InChIKeyhelp_outline XLYOFNOQVPJJNP-UHFFFAOYSA-N SMILEShelp_outline [H]O[H] 2D coordinates Mol file for the small molecule Search links Involved in 6,264 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Cross-references
| RHEA:20920 | RHEA:20921 | RHEA:20922 | RHEA:20923 | |
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| Reaction direction help_outline | undefined | left-to-right | right-to-left | bidirectional |
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Publications
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The purification and properties of 2,4-dichlorophenol hydroxylase from a strain of Acinetobacter species.
Beadle C.A., Smith A.R.
1. 2,4-Dichlorophenol hydroxylase has been purified 13-fold from Acinetobacter grown on 2,4-dichlorophenoxyacetic acid as sole carbon source. The enzyme was estimated to be 80-90% pure by electrophoresis. 2. The enzyme has a relative molecular mass of about 240 000 and consists of four subunits of ... >> More
1. 2,4-Dichlorophenol hydroxylase has been purified 13-fold from Acinetobacter grown on 2,4-dichlorophenoxyacetic acid as sole carbon source. The enzyme was estimated to be 80-90% pure by electrophoresis. 2. The enzyme has a relative molecular mass of about 240 000 and consists of four subunits of identical size. 3. The enzyme contains FAD as the prosthetic group. FAD could not be replaced by riboflavin or FMN in reconstituting active enzyme from apoenzyme. 4. The reaction catalysed is an NADPH-dependent hydroxylation of 2,4-dichlorophenol with the formation of 3,5-dichlorocatechol as product. The reaction stoichiometry is typical of a monooxygenase with an external electron donor. NADPH is the preferred reduced pyridine nucleotide substrate but the enzyme can function with NADH. 5. The enzyme possesses broad effector specificity. In addition to 2,4-dichlorophenol, 4-chlorophenol and 4-chloro-2-methylphenol are true substrates for the enzyme. A number of 'non-substrate effectors' has been found. 6. The enzyme is sensitive to thiol-inhibiting reagents. << Less