Enzymes
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- Name help_outline juglone Identifier CHEBI:15794 (CAS: 481-39-0) help_outline Charge 0 Formula C10H6O3 InChIKeyhelp_outline KQPYUDDGWXQXHS-UHFFFAOYSA-N SMILEShelp_outline Oc1cccc2C(=O)C=CC(=O)c12 2D coordinates Mol file for the small molecule Search links Involved in 2 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline O2 Identifier CHEBI:15379 (CAS: 7782-44-7) help_outline Charge 0 Formula O2 InChIKeyhelp_outline MYMOFIZGZYHOMD-UHFFFAOYSA-N SMILEShelp_outline O=O 2D coordinates Mol file for the small molecule Search links Involved in 2,779 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline 3,5-dihydroxy-1,4-naphthoquinone Identifier CHEBI:58026 Charge -1 Formula C10H5O4 InChIKeyhelp_outline VYGYXAIGQZWAQC-UHFFFAOYSA-M SMILEShelp_outline Oc1cccc2C(=O)C=C([O-])C(=O)c12 2D coordinates Mol file for the small molecule Search links Involved in 2 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline H+ Identifier CHEBI:15378 Charge 1 Formula H InChIKeyhelp_outline GPRLSGONYQIRFK-UHFFFAOYSA-N SMILEShelp_outline [H+] 2D coordinates Mol file for the small molecule Search links Involved in 9,717 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Cross-references
RHEA:18745 | RHEA:18746 | RHEA:18747 | RHEA:18748 | |
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Reaction direction help_outline | undefined | left-to-right | right-to-left | bidirectional |
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Publications
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Purification and some properties of two isofunctional juglone hydroxylases from Pseudomonas putida J1.
Rettenmaier H., Lingens F.
Juglone-induced cells of Pseudomonas putida J 1 were shown to contain two isofunctional juglone hydroxylases. Both enzymes were purified about 125-fold to homogeneity in polyacrylamide gel electrophoresis. The molecular masses of the native enzymes, as determined by Sephacryl S-200 gel filtration ... >> More
Juglone-induced cells of Pseudomonas putida J 1 were shown to contain two isofunctional juglone hydroxylases. Both enzymes were purified about 125-fold to homogeneity in polyacrylamide gel electrophoresis. The molecular masses of the native enzymes, as determined by Sephacryl S-200 gel filtration were 59 000 Da for enzyme 1 and 56 000 Da for enzyme 2. The molecular masses of the subunits were determined by dodecyl sulfate polyacrylamide gel electrophoresis as 25 000 Da (enzyme 1) and 23 500 Da (enzyme 2). Both enzymes hydroxylated juglone, naphthazarin, 1,4-naphthoquinone and 2-chloro-1,4-naphthoquinone, but they were completely inactive against naphtholes. The activity of both hydroxylases was not affected by chelating agents, thiol reagents, however, were found to be strong inhibitors. No external cofactors such as Fe2, NADH, NADPH, FAD, FMN were required for activity. Concomitant with the hydroxylation of juglone the consumption of oxygen in a molar ratio 2: 1 (juglone: oxygen) was observed but none of the enzymes incorporated 18O2 into the substrate juglone. By activity staining enzyme 1 was found to be present in induced and non-induced cells of P. putida J 1, enzyme 2, however, only in juglone-induced cells. << Less
Biol Chem Hoppe Seyler 366:637-646(1985) [PubMed] [EuropePMC]
This publication is cited by 2 other entries.
Comments
Multi-step reaction: RHEA:50748 and RHEA:50752.