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- Name help_outline N-sulfo-D-glucosamine Identifier CHEBI:57868 Charge -1 Formula C6H12NO8S InChIKeyhelp_outline PRDZVHCOEWJPOB-IVMDWMLBSA-M SMILEShelp_outline OC[C@H]1OC(O)[C@H](NS([O-])(=O)=O)[C@@H](O)[C@@H]1O 2D coordinates Mol file for the small molecule Search links Involved in 2 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline H2O Identifier CHEBI:15377 (CAS: 7732-18-5) help_outline Charge 0 Formula H2O InChIKeyhelp_outline XLYOFNOQVPJJNP-UHFFFAOYSA-N SMILEShelp_outline [H]O[H] 2D coordinates Mol file for the small molecule Search links Involved in 6,264 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline D-glucosamine Identifier CHEBI:58723 Charge 1 Formula C6H14NO5 InChIKeyhelp_outline MSWZFWKMSRAUBD-IVMDWMLBSA-O SMILEShelp_outline [NH3+][C@H]1C(O)O[C@H](CO)[C@@H](O)[C@@H]1O 2D coordinates Mol file for the small molecule Search links Involved in 9 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline sulfate Identifier CHEBI:16189 (CAS: 14808-79-8) help_outline Charge -2 Formula O4S InChIKeyhelp_outline QAOWNCQODCNURD-UHFFFAOYSA-L SMILEShelp_outline [O-]S([O-])(=O)=O 2D coordinates Mol file for the small molecule Search links Involved in 91 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Cross-references
| RHEA:17881 | RHEA:17882 | RHEA:17883 | RHEA:17884 | |
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| Reaction direction help_outline | undefined | left-to-right | right-to-left | bidirectional |
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Publications
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A rapid four column purification of 2-deoxy-D-glucoside-2-sulphamate sulphohydrolase from human liver.
Mahuran D., Clements P., Hopwood J.
2-Deoxy-D-glucoside-2-sulphamate sulphohydrolase was extracted from human liver and purified 40 000-fold by a simple four column procedure. The purification was followed using a specific substrate isolated from an acid hydrolysate of heparin, O-(alpha-2-sulphamino-2-deoxy-D-glucopyranosyl)-(1 lead ... >> More
2-Deoxy-D-glucoside-2-sulphamate sulphohydrolase was extracted from human liver and purified 40 000-fold by a simple four column procedure. The purification was followed using a specific substrate isolated from an acid hydrolysate of heparin, O-(alpha-2-sulphamino-2-deoxy-D-glucopyranosyl)-(1 leads to 3)-L-[6,3H]idonic acid. Only one form of the enzyme was seen on either ion exchange chromatography or isoelectric focussing, with a pI of 6.8. The apparent Mr of the holoenzyme as determined by gel filtration was 190 000 +/-20 000. Two other larger Mr protein peaks observed on gel filtration appear to be an inactive dimer of the 190 000 dalton peak and a larger aggregate near the exclusion limit of the column. On polyacrylamide disc gel electrophoresis in sodium dodecyl sulphate, with or without prior reduction, each protein peak from the gel filtration column electrophoresed as a single major band with an apparent Mr corresponding to 55 000 +/- 6000. << Less
Comments
Reference: Structure of sulfamidase provides insight into the molecular pathology of mucopolysaccharidosis IIIA Sidhu Navdeep S., Schreiber Kathrin, Proepper Kevin, Becker Stefan, Uson Isabel, Sheldrick George M., Gaertner Jutta, Kraetzner Ralph, Steinfeld Robert Structure of sulfamidase provides insight into the molecular pathology of mucopolysaccharidosis IIIA Sidhu Navdeep S., Schreiber Kathrin, Proepper Kevin, Becker Stefan, Uson Isabel, Sheldrick George M., Gaertner Jutta, Kraetzner Ralph, Steinfeld Robert ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY 70, 1321-1335 (2014). DOI: 10.1107/S1399004714002739