Enzymes
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- Name help_outline AMP Identifier CHEBI:456215 Charge -2 Formula C10H12N5O7P InChIKeyhelp_outline UDMBCSSLTHHNCD-KQYNXXCUSA-L SMILEShelp_outline Nc1ncnc2n(cnc12)[C@@H]1O[C@H](COP([O-])([O-])=O)[C@@H](O)[C@H]1O 2D coordinates Mol file for the small molecule Search links Involved in 508 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline thymidine Identifier CHEBI:17748 (Beilstein: 89285; CAS: 50-89-5) help_outline Charge 0 Formula C10H14N2O5 InChIKeyhelp_outline IQFYYKKMVGJFEH-XLPZGREQSA-N SMILEShelp_outline Cc1cn([C@H]2C[C@H](O)[C@@H](CO)O2)c(=O)[nH]c1=O 2D coordinates Mol file for the small molecule Search links Involved in 11 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline adenosine Identifier CHEBI:16335 (Beilstein: 93029; CAS: 58-61-7) help_outline Charge 0 Formula C10H13N5O4 InChIKeyhelp_outline OIRDTQYFTABQOQ-KQYNXXCUSA-N SMILEShelp_outline Nc1ncnc2n(cnc12)[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O 2D coordinates Mol file for the small molecule Search links Involved in 21 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline dTMP Identifier CHEBI:63528 (Beilstein: 3916216) help_outline Charge -2 Formula C10H13N2O8P InChIKeyhelp_outline GYOZYWVXFNDGLU-XLPZGREQSA-L SMILEShelp_outline Cc1cn([C@H]2C[C@H](O)[C@@H](COP([O-])([O-])=O)O2)c(=O)[nH]c1=O 2D coordinates Mol file for the small molecule Search links Involved in 14 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Cross-references
| RHEA:14913 | RHEA:14914 | RHEA:14915 | RHEA:14916 | |
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| Reaction direction help_outline | undefined | left-to-right | right-to-left | bidirectional |
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Publications
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Adenylic acid: deoxythymidine 5'-phosphotransferase: evidence for the existence of a novel herpes simplex virus-induced enzyme.
Falke D., Nehrbass W., Brauer D., Muller W.E.
BHK (dPyK-) cells infected with herpes simplex virus type 1 (HSV-1) contain a virus-induced deoxythymidine (dThd)-phosphorylating enzyme. This enzyme uses AMP as phosphate donor and is called AMP :deoxythymidine 5'-phosphotransferase (or kinase). The enzyme was purified over 1300-fold and was foun ... >> More
BHK (dPyK-) cells infected with herpes simplex virus type 1 (HSV-1) contain a virus-induced deoxythymidine (dThd)-phosphorylating enzyme. This enzyme uses AMP as phosphate donor and is called AMP :deoxythymidine 5'-phosphotransferase (or kinase). The enzyme was purified over 1300-fold and was found to be specific for an AMP substrate. It can thus be distinguished from virus-specific deoxypyrimidine kinase (dPyK). It is shown that the two substrates AMP and dThd participate in the reaction at a 1 : 1 molar ratio; the Km for AMP was 2 X 3 muM and for dThd it was 2 X 1 muM. The mol. wt. of the enzyme was estimated to be between 110 000 (by glycerol gradient centrifugation) and 90 000 (by gel filtration). For optimum activity, the phosphotransferase required an alkaline pH, and 37 degrees C; the activation energy of the reaction was 18 450 cal/mol. The appearance of the enzyme after infection parallels that of viral DNA synthesis-related functions. << Less
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Adenosine diphosphate: thymidine 5'-phosphotransferase, a new enzyme activity, associated with the Herpes simplex virus-induced deoxypyrimidine kinase.
Falke D., Labenz J., Brauer D., Muller W.E.
The deoxypyrimidine kinase induced in mouse fibroblasts, strain CLID (TK-) infected with either herpes simplex virus (HSV) type 1 or type 2, possesses besides deoxypyrimidine kinase (ATP:dThd/dCyd phosphotransferase) two further enzyme activities: an AMP:dThd phosphotransferase and an ADP:dThd pho ... >> More
The deoxypyrimidine kinase induced in mouse fibroblasts, strain CLID (TK-) infected with either herpes simplex virus (HSV) type 1 or type 2, possesses besides deoxypyrimidine kinase (ATP:dThd/dCyd phosphotransferase) two further enzyme activities: an AMP:dThd phosphotransferase and an ADP:dThd phosphotransferase. The latter enzyme activity, described in this report, was found to be inhibited by antiserum against the HSV deoxypyrimidine kinase and to be absent after infection with TK-mutant MDK 10 (B 2006). The ADP:dThd phosphotransferase, which had been purified approx. 340-fold, differs by a series of physicochemical properties from the viral AMP:dThd- and ATP:dThd phosphotransferase. << Less
Biochim Biophys Acta 708:99-103(1982) [PubMed] [EuropePMC]
This publication is cited by 2 other entries.