Enzymes
| UniProtKB help_outline | 9,329 proteins |
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| GO Molecular Function help_outline |
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Reaction participants Show >> << Hide
- Name help_outline D-glucarate Identifier CHEBI:30612 (Beilstein: 3909239) help_outline Charge -2 Formula C6H8O8 InChIKeyhelp_outline DSLZVSRJTYRBFB-LLEIAEIESA-L SMILEShelp_outline O[C@@H]([C@H](O)[C@@H](O)C([O-])=O)[C@H](O)C([O-])=O 2D coordinates Mol file for the small molecule Search links Involved in 7 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline 5-dehydro-4-deoxy-D-glucarate Identifier CHEBI:42819 Charge -2 Formula C6H6O7 InChIKeyhelp_outline QUURPCHWPQNNGL-ZAFYKAAXSA-L SMILEShelp_outline O[C@@H](CC(=O)C([O-])=O)[C@@H](O)C([O-])=O 2D coordinates Mol file for the small molecule Search links Involved in 7 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline H2O Identifier CHEBI:15377 (CAS: 7732-18-5) help_outline Charge 0 Formula H2O InChIKeyhelp_outline XLYOFNOQVPJJNP-UHFFFAOYSA-N SMILEShelp_outline [H]O[H] 2D coordinates Mol file for the small molecule Search links Involved in 6,264 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Cross-references
| RHEA:14573 | RHEA:14574 | RHEA:14575 | RHEA:14576 | |
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| Reaction direction help_outline | undefined | left-to-right | right-to-left | bidirectional |
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Publications
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New insights into the alternative D-glucarate degradation pathway.
Aghaie A., Lechaplais C., Sirven P., Tricot S., Besnard-Gonnet M., Muselet D., de Berardinis V., Kreimeyer A., Gyapay G., Salanoubat M., Perret A.
Although the D-glucarate degradation pathway is well characterized in Escherichia coli, genetic and biochemical information concerning the alternative pathway proposed in Pseudomonas species and Bacillus subtilis remains incomplete. Acinetobacter baylyi ADP1 is a Gram-negative soil bacterium posse ... >> More
Although the D-glucarate degradation pathway is well characterized in Escherichia coli, genetic and biochemical information concerning the alternative pathway proposed in Pseudomonas species and Bacillus subtilis remains incomplete. Acinetobacter baylyi ADP1 is a Gram-negative soil bacterium possessing the alternative pathway and able to grow using D-glucarate as the only carbon source. Based on the annotation of its sequenced genome (1), we have constructed a complete collection of singlegene deletion mutants (2). High throughput profiling for growth on a minimal medium containing D-glucarate as the only carbon source for approximately 2450 mutants led to the identification of the genes involved in D-glucarate degradation. Protein purification after recombinant production in E. coli allowed us to reconstitute the enzymatic pathway in vitro. We describe here the kinetic characterization of D-glucarate dehydratase, d-5-keto-4-deoxyglucarate dehydratase, and of cooperative alpha-ketoglutarate semialdehyde dehydrogenase. Transcription and expression analyses of the genes involved in D-glucarate metabolism within a single organism made it possible to access information regarding the regulation of this pathway for the first time. << Less
J. Biol. Chem. 283:15638-15646(2008) [PubMed] [EuropePMC]
This publication is cited by 2 other entries.