Rhea - reaction knowledgebase

Enzymes

UniProtKB ^help_outline	1 proteins
Enzyme class ^help_outline	EC 1.5.8.1 dimethylamine dehydrogenase
GO Molecular Function ^help_outline	GO:0047133 dimethylamine dehydrogenase activity

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Name ^help_outline dimethylamine Identifier CHEBI:58040 Charge 1 Formula C2H8N InChIKey^help_outline ROSDSFDQCJNGOL-UHFFFAOYSA-O SMILES^help_outline C[NH2+]C 2D coordinates Mol file for the small molecule Search links Involved in 8 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Name ^help_outline H⁺ Identifier CHEBI:15378 Charge 1 Formula H InChIKey^help_outline GPRLSGONYQIRFK-UHFFFAOYSA-N SMILES^help_outline [H+] 2D coordinates Mol file for the small molecule Search links Involved in 9,431 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Name ^help_outline H₂O Identifier CHEBI:15377 (Beilstein: 3587155; CAS: 7732-18-5) ^help_outline Charge 0 Formula H2O InChIKey^help_outline XLYOFNOQVPJJNP-UHFFFAOYSA-N SMILES^help_outline [H]O[H] 2D coordinates Mol file for the small molecule Search links Involved in 6,204 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Name^help_outline oxidized [electron-transfer flavoprotein] Identifier RHEA-COMP:10685 Reactive part ^help_outline
- Name ^help_outline FAD Identifier CHEBI:57692 Charge -3 Formula C27H30N9O15P2 InChIKey^help_outline IMGVNJNCCGXBHD-UYBVJOGSSA-K SMILES^help_outline Cc1cc2nc3c(nc(=O)[n-]c3=O)n(C[C@H](O)[C@H](O)[C@H](O)COP([O-])(=O)OP([O-])(=O)OC[C@H]3O[C@H]([C@H](O)[C@@H]3O)n3cnc4c(N)ncnc34)c2cc1C 2D coordinates Mol file for the small molecule Search links Involved in 170 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Search links Involved in 64 reaction(s) Find proteins in UniProtKB for this molecule
Name ^help_outline formaldehyde Identifier CHEBI:16842 (Beilstein: 1209228; CAS: 50-00-0) ^help_outline Charge 0 Formula CH2O InChIKey^help_outline WSFSSNUMVMOOMR-UHFFFAOYSA-N SMILES^help_outline [H]C([H])=O 2D coordinates Mol file for the small molecule Search links Involved in 141 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Name ^help_outline methylamine Identifier CHEBI:59338 Charge 1 Formula CH6N InChIKey^help_outline BAVYZALUXZFZLV-UHFFFAOYSA-O SMILES^help_outline C[NH3+] 2D coordinates Mol file for the small molecule Search links Involved in 27 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Name^help_outline reduced [electron-transfer flavoprotein] Identifier RHEA-COMP:10686 Reactive part ^help_outline
- Name ^help_outline FADH₂ Identifier CHEBI:58307 Charge -2 Formula C27H33N9O15P2 InChIKey^help_outline YPZRHBJKEMOYQH-UYBVJOGSSA-L SMILES^help_outline Cc1cc2Nc3c([nH]c(=O)[nH]c3=O)N(C[C@H](O)[C@H](O)[C@H](O)COP([O-])(=O)OP([O-])(=O)OC[C@H]3O[C@H]([C@H](O)[C@@H]3O)n3cnc4c(N)ncnc34)c2cc1C 2D coordinates Mol file for the small molecule Search links Involved in 161 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Search links Involved in 64 reaction(s) Find proteins in UniProtKB for this molecule

Cross-references

	RHEA:10204	RHEA:10205	RHEA:10206	RHEA:10207
Reaction direction	undefined	left-to-right	right-to-left	bidirectional
UniProtKB ^help_outline	1 proteins
EC numbers ^help_outline	1.5.8.1
Gene Ontology ^help_outline	GO:0047133
KEGG ^help_outline				R01588
MetaCyc ^help_outline	1.5.8.1-RXN

Publications

The primary structure of Hyphomicrobium X dimethylamine dehydrogenase. Relationship to trimethylamine dehydrogenase and implications for substrate recognition.

Yang C.C., Packman L.C., Scrutton N.S.

The gene encoding dimethylamine dehydrogenase from Hyphomicrobium X has been cloned and over-expressed in Escherichia coli. Using the chemically determined protein sequence, primers were designed to amplify DNA fragments encoding the proximal and distal parts of the gene. These fragments were used ... >> More

The gene encoding dimethylamine dehydrogenase from Hyphomicrobium X has been cloned and over-expressed in Escherichia coli. Using the chemically determined protein sequence, primers were designed to amplify DNA fragments encoding the proximal and distal parts of the gene. These fragments were used to synthesise two probes and the dmd gene was cloned as part of two BamHI fragments isolated from digested genomic DNA. The sequence of the complete open reading frame was determined on both strands and contained 2211 bp coding for a protein of 736 amino acids, including the N-terminal methionine residue that is removed when expressed in the native host. The molecular mass of the processed apoprotein predicted from the DNA sequence is 82,523 Da. Dimethylamine dehydrogenase is closely related to the trimethylamine dehydrogenase of Methylophilus methylotrophus W3A1 (63.5% identical) and other class I FMN-binding beta 8 alpha 8 barrel flavoproteins. Residues in the active site of trimethylamine dehydrogenase that are known, or implicated, to be important in catalysis are conserved in dimethylamine dehydrogenase. Sequence alignment of dimethylamine and trimethylamine dehydrogenases suggests that the specificity for secondary and tertiary amines resides in a single amino acid substitution in a substrate-binding aromatic bowl located in the active site of the enzymes. << Less

Eur. J. Biochem. 232:264-271(1995) [PubMed] [EuropePMC]

RHEA:10204 dimethylamine + H(+) + H2O + oxidized [electron-transfer flavoprotein] = formaldehyde + methylamine + reduced [electron-transfer flavoprotein]

Enzymes

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Cross-references

Publications

The primary structure of Hyphomicrobium X dimethylamine dehydrogenase. Relationship to trimethylamine dehydrogenase and implications for substrate recognition.